EMAIL THIS PAGE TO A FRIEND
97622 Supelco

2-Mercaptoethanol

for HPLC derivatization, LiChropur, ≥99.0% (GC)

Synonym: β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

  • CAS Number 60-24-2

  • Linear Formula HSCH2CH2OH

  • Molecular Weight 78.13

  •  Beilstein/REAXYS Number 773648

  •  EC Number 200-464-6

  •  MDL number MFCD00004890

  •  PubChem Substance ID 329770550

  •  NACRES NA.05

Purchase

Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Derivatization Reagents, Derivatization Reagents HPLC, Mass Spectrometry More...
Quality Level   100
grade   for HPLC derivatization
vapor density   2.69 (vs air)
vapor pressure   1 mmHg ( 20 °C)
assay   ≥99.0% (GC)
quality   LiChropur
expl. lim.   18 %
concentration   14.3 M (pure liquid)
application(s)   HPLC: suitable
refractive index   n20/D 1.500 (lit.)
  n20/D 1.500-1.502
bp   157 °C (lit.)
density   1.114 g/mL at 25 °C (lit.)
storage temp.   2-8°C
SMILES string   OCCS
InChI   1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
InChI key   DGVVWUTYPXICAM-UHFFFAOYSA-N

Description

General description

2-Mercaptoethanol is a thiol compound showing growth promoting properties on mouse lymphoma L1210 cells in vitro. It also enhances viability, blast transformation and antibody formation in lymphocyte cultures.

Application

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

suitable for derivatisation of Amine groups

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Signal word 
Danger
RIDADR 
UN 2966 6.1 / PGII
WGK Germany 
WGK 3
RTECS 
KL5600000
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?