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C5070 Sigma-Aldrich

CHAPS hydrate

BioXtra, ≥98% (TLC)

Synonym: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS

  • CAS Number 331717-45-4

  • Empirical Formula (Hill Notation) C32H58N2O7S · xH2O

  • Molecular Weight 614.88 (anhydrous basis)

  •  PubChem Substance ID 24892750

  •  NACRES NA.21

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Core Bioreagents, Detergents,
Quality Level   400
description   zwitterionic
product line   BioXtra
assay   ≥98% (TLC)
mol wt   614.88 g/mol (anhydrous basis)
application(s)   protein purification: suitable
  protein quantification: suitable
impurities   ≤0.001% Phosphorus (P)
  ≤0.1% Insoluble matter
ign. residue   ≤0.1%
mp   157 °C (dec.) (lit.)
solubility   H2O: 0.1 M, clear, colorless
anion traces   chloride (Cl-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Na: ≤0.005%
  Pb: ≤0.001%
  Zn: ≤0.0005%
SMILES string   O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C
InChI   1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1
InChI key   SJCUTFKCLFLIFE-JWTJKVBLSA-N

Description

General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

• 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
• 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
• HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.

Packaging

1, 5 g in poly bottle

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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