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D9015 Sigma-Aldrich

3,3′-Diaminobenzidine tetrahydrochloride hydrate

ISOPAC®

  • CAS Number 868272-85-9

  • Empirical Formula (Hill Notation) C12H14N4 · 4HCl · xH2O

  • Molecular Weight 360.11 (anhydrous basis)

  •  EC Number 231-018-9

  •  MDL number MFCD08273058

  •  PubChem Substance ID 24894241

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Description

Caution

Injecting a compatible solvent permits preparation of any desired strength solution without exposure.

Packaging

100 mg in serum bottle

Weight approximate. Packaged in a 100 mL serum bottle with silicon/PTFE septum and aluminum tear seal.

Reconstitution

Dissolving the contents in 100 mL of solvent yields a 0.1% solution.

Application

3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridization.

Biochem/physiol Actions

DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.

Legal Information

Isopac is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
DV8753000

Mitigate Risk with Diagnostic Elite
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

References

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D5637 3,3′-Diaminobenzidine tetrahydrochloride hydrate, ≥96%

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