EMAIL THIS PAGE TO A FRIEND
A0169 Sigma-Aldrich

Agarose

Type I-A, low EEO

Purchase

Properties

Related Categories Agarose, Core Bioreagents, Life Science Reagents for Cloning, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Northern and Southern Blotting,
Quality Level   200
type   Type I-A
form   powder
impurities   ≤7% water
ash   ≤0.5%
turbidity   ≤4 NTU (1.5% gel)
EEO   0.09-0.13
mp   86.5-89.5  °C
transition temp   gel point 36 °C ±1.5 °C (1.5% gel)
gel strength   ≥1200 g/cm2 (1% gel)
anion traces   sulfate (SO42-): ≤0.20%
InChI   1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key   MJQHZNBUODTQTK-WKGBVCLCSA-N

Description

General description

Agarose, a natural polymer is obtained from sea algae. This neutral linear polysaccharide is made up of d-galactose and 3,6-anhydro-l-galactose. These are connected alternatively with the help of glycosidic bonds. Agarose has high significance in gel electrophoresis and gel chromatography.

Application

Agarose has been used:
• as a component in the preparation of tumor tissue to embed the cell material
• in the sample preparation for quantum dot analysis
• in the preparation of agarose-solidified medium to study the effect of medium solidification on the culture of isolated ovules

Packaging

1 kg in poly bottle

10, 25, 50, 100, 250, 500 g in poly bottle

Biochem/physiol Actions

Agarose is used as bioink, because of its characteristic properties such as, biocompatibility, mechanical strength and gelling ability at low temperature.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles

Related Content

Agarose Selection Guide

Agarose Selection Guide – Click on the Product Number to Purchase Specialty Agarose – Click on the Product Number to Purchase
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?