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A4018 Sigma-Aldrich

Agarose, low gelling temperature

Synonym: 2-Hydroxyethyl agarose

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Properties

Related Categories Agarose, Core Bioreagents, Molecular Biology, Nucleic Acid Electrophoresis, Research Essentials More...
Quality Level   200
biological source   algae (marine)
application(s)   cell culture | mammalian: suitable
EEO   ≤0.10
transition temp   congealing temperature 26-30 °C
gel strength   ≥200 g/cm2 (1% gel)

Description

General description

Agarose is a polymer extracted from agar or agar-bearing marine algae. This purified linear galactan hydrocolloid comprises alternating co-polymers D-galactose and 3,6-anhydro-L-galactose units connected by α-(1→3) and β-(1→4) glycosidic bonds. Agarose is highly biocompatible and possesses variable mechanical and diffusion properties. Agarose can be used as a gelling agent, to separate nucleic acids electrophoretically. Low melting or low gelling temperature agarose is produced by hydroxyethylation of agarose. It is usually used for the isolation of separated DNA fragments. DNA fragments of the equal size will take longer time to move through a low melting agarose gel compared to a standard agarose gel.

Application

Gels exhibit excellent clarity and are particularly useful for the preparation of media containing heat-labile materials. Recommended for preparation of agarose beads.
Agarose, low gelling temperature has been used:
• to immobilize zebra fish embryos during in vivo imaging experiments
• as a constituent of defined medium to culture bovine nucleus pulposus (NP) cells
• as a hydrogel, a scaffolding material for dental pulp regeneration
• in the form of pellets to determine the surface pH and conductivity of an acrylic emulsion film

Packaging

1, 5, 10, 25, 50, 100 g in poly bottle

Quality

A low gelling temperature derivative with unique gelling properties.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Agarose Product Information

CAS Number: 9012-36-6 (product A9539) CAS Number: 39346-81-1 (products A9414, A4018, A6560, A9045, A0701) Synonyms: 3,6-Anhydro-α-L-galacto-β-D-galactan; FastLane agarose; Indubiose A4; NuSieve GTG; ...
Keywords: Affinity chromatography, Cell culture, Chromatography, Cloning, Counterimmunoelectrophoresis, Culture media, DNA electrophoresis, Diffusion, Electrophoresis, Gel electrophoresis, Immunodiffusion, Immunoelectrophoresis, Indicators, Ion Exchange, Isoelectric focusing, Melting, Molecular biology, Polymerase chain reaction, Purification, Sample preparations, Separation

Peer-Reviewed Papers
15

References

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