EMAIL THIS PAGE TO A FRIEND
A6013 Sigma-Aldrich

Agarose

Type I, low EEO

Purchase

Properties

Related Categories Agarose, Core Bioreagents, Life Science Reagents for Cloning, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Northern and Southern Blotting,
Quality Level   200
biological source   algae (Gelidium & Gracilaria)
type   Type I
form   powder
application(s)   electrophoresis: suitable
impurities   ≤10% water
EEO   0.09-0.13
transition temp   gel point 36 °C ±1.5 °C (1.5% gel)
gel strength   ≥1200 g/cm2 (1% gel)
anion traces   sulfate (SO42-): ≤0.15%
InChI   1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key   MJQHZNBUODTQTK-WKGBVCLCSA-N

Description

General description

Agarose is a polymer of agarobiose (L- and D-galactose) subunits. It is isolated from the seaweed Gelidium and Gracilaria. Agarose forms non-covalent network of bundles. It is exploited for the separation of nucleic acids. The concentration and pore size of the gel are inversely related. Agarose has wide range of separation and is simple and rapid to prepare without the need of catalyst.

Application

Agarose has been used:
• in the embedding of fluorescent nanospheres.
• for the generation of tissue-like phantoms similar to myocardial tissues.
• in hydrogel preparation.

Packaging

1 kg in poly bottle

5, 10, 25, 50, 100, 250, 500 g in poly bottle

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

pHast Pack
Protocols & Articles

Related Content

Agarose Selection Guide

Agarose Selection Guide – Click on the Product Number to Purchase Specialty Agarose – Click on the Product Number to Purchase
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation

Agriculture | Crop Protection Workflow

Pesticides are an essential component in modern agriculture and enable growers to fully realize the yield potential of their seeds. From compound discovery to active ingredient development, our chemi...
Keywords: Agriculture, Building blocks, Gas chromatography, Gas chromatography mass spectrometry, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Organic synthesis, Pesticides, Polymerase chain reaction - quantitative, Purification, Solvents

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?