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A7906 Sigma-Aldrich

Bovine Serum Albumin

heat shock fraction, pH 7, ≥98%

Synonym: Albumin bovine serum, BSA, Bovine albumin

Purchase

Properties

Related Categories Albumin, Biochemicals and Reagents, Bovine Serum Albumins, Core Bioreagents, General Reagents,
biological source   bovine
assay   ≥98%
form   lyophilized powder
mol wt   ~66 kDa
purified by   heat shock fractionation
origin   USA origin
loss   ≤5%
pH   7
solubility   water: soluble (40 mg/ml)
storage temp.   2-8°C

Description

Application

Bovine serum albumin has been used-
• as a component of PCR (polymerase chain reaction) mix
• in the preparation of dissociated cortical neurons
• during immunofluorescent labeling
• as a component of Ca2+-free saline (containing collagenase, hyaluronidase and DNAse) used for bovine tissue digestion

Packaging

1, 5, 10 kg in poly drum

10, 50, 100, 500 g in poly bottle

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Preparation Note

Prepared using heat shock fractionation.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

IsProduct A7906, Albumin from bovine serum (BSA), suitable for use as a blocking agent in ELISA?
We have not tested the use of A7906 in ELISA. Because the remainder of the product is mostly globulins, we would not suggest A7906 for ELISA use.
Is Product A7906, Albumin from bovine serum (BSA), protease-free?
We have not tested A7906 in quality control to be protease-free. Other BSA products to suggest that are protease-free would be A3059 or B6917.
Can Product A7906, Albumin from bovine serum (BSA), be used in immunohistochemistry?
We have not ourselves tested A7906 for use in immunohistochemistry. However, a literature search can turn up publications that cite this use, such as this paper: Surendran, K., et al., Wnt-Dependent Beta-Catenin Signaling Is Activated after Unilateral Ureteral Obstruction, and Recombinant Secreted Frizzled-Related Protein 4 Alters the Progression of Renal Fibrosis. J. Am. Soc. Nephrol., Vol. 16, pp. 2373-2384 (2005).
IsProduct A7906, Albumin from bovine serum (BSA), tested for insulin levels?
A7906 is not tested in quality control for insulin levels. One BSA that is indicated as "suitable for insulin RIA procedures" is A7888.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles
Peer-Reviewed Papers
15

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