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  • A8456 - 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride

A8456 Sigma-Aldrich

4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride

≥97.0% (HPLC)

Synonym: AEBSF

  • CAS Number 30827-99-7

  • Empirical Formula (Hill Notation) C8H10FNO2S · HCl

  • Molecular Weight 239.69

  •  Beilstein/REAXYS Number 7604627

  •  MDL number MFCD00132962

  •  PubChem Substance ID 24278240

  •  NACRES NA.77



Related Categories A to C, Biochemicals and Reagents, Broad Spectrum Inhibitors of Proteolytic Enzyme Classes, Chymotrypsin, α-, Chymotrypsin, alpha-,
Quality Level   300
biological source   synthetic (organic)
assay   ≥97.0% (HPLC)
form   powder
mp   183-191  °C
solubility   H2O: 50 mg/mL (stable for up to six months if stored refrigerated at a pH of less than 7. If a pH of greater than 7 is required, pH adjustment should be made just prior to use.)
storage temp.   −20°C
SMILES string   FS(C1=CC=C(CCN)C=C1)(=O)=O.[H]Cl
InChI   1S/C8H10FNO2S.ClH/c9-13(11,12)8-3-1-7(2-4-8)5-6-10;/h1-4H,5-6,10H2;1H



25, 100, 500 mg in poly bottle


Recommended concentrations for use are 0.1-1 mM.

Biochem/physiol Actions

Irreversible serine protease inhibitor. Inhibition constants are similar to those of PMSF and DFP. AEBSF has been shown to inhibit trypsin, chymotrypsin, plasmin kallikrein and thrombin. As an alternative to PMSF and DFP, AEBSF offers lower toxicity, improved solubility in water and improved stability in aqueous solutions. AEBSF has been used in cell culture in concentrations of up to 0.25 mM.

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
In what solvents is Product A8456, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, soluble?
It is highly soluble in water and in DMSO, at 50 mg/mL (approximately 200 mM), yielding a clear colorless solution. AEBSF may be used in cell culture medium at concentrations up to 0.25 mM.
How stable is Product A8456, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride, in solution?
Solutions in water are slightly acidic and retain inhibitory activity for up to six months when stored refrigerated. Solutions at pHs above 7 are less stable.Stock solutions should be stored at a pH less than 7. If a final pH of greater than 7 is required, the pH adjustment should be done shortly before use.
What is the recommended working concentration for Product A8456, 4-(2-Aminoethyl)benzenesulfonyl fluoride hydrochloride?
Our recommended working concentration range is 0.1 mM to 1 mM; the optimal concentration will depend on the application.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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