• USA Home
  • B2261 - bisBenzimide H 33342 trihydrochloride

EMAIL THIS PAGE TO A FRIEND
B2261 Sigma-Aldrich

bisBenzimide H 33342 trihydrochloride

≥98% (HPLC and TLC)

Synonym: 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide

  • CAS Number 23491-52-3

  • Empirical Formula (Hill Notation) C27H28N6O · 3HCl · xH2O

  • Molecular Weight 561.93 (anhydrous basis)

  •  Beilstein/REAXYS Number 1234011

  •  MDL number MFCD00012678

  •  PubChem Substance ID 24891639

Purchase

Properties

Related Categories Alphabetical, Apoptosis Detection, Apoptosis and Cell Cycle, B, Biochemicals and Reagents,
InChI Key   FYEVKHPLBHLWHK-UHFFFAOYSA-N
Quality Level   PREMIUM
assay   ≥98% (HPLC and TLC)
solubility   H2O: 20 mg/mL
  phosphate buffer: precipitates
suitability   suitable for fluorescence
storage temp.   −20°C

Description

Packaging

1 g in glass bottle

100, 500 mg in glass bottle

25 mg in poly bottle

Application

Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

bisBenzimide H 33342 trihydrochloride has been used for the staining of viable and apoptotic cells. It has been used for the staining of nuclei in cells, which were analyzed by flow cytometry.

Biochem/physiol Actions

Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Certificate of Origin

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is a typical concentration of Product B2261, bisBenzimide H 33342 trihydrochloride,for staining DNA in cells?
200 micrograms per mL.
Can solvents other than water be used to dissolve Product B2261, bisBenzimide H 33342 trihydrochloride?
Hoechst 33342 can be dissolved in N,N-dimethylformamide (DMF).
Which Hoechst dye is suitable for chromosomal analysis?
Hoechst 33258 (Product No. B2883) is the preferred Hoechst dye for chromosomal analysis. Hoechst 33342 (Product No. B2261) is a poor fluorochrome for chromosomal analysis.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

ABC Transporters and Cancer Drug Resistance

Chemotherapy is the treatment of choice against many types of cancer. However, over time chemotherapeutic drugs can become less effective due to the development of resistance that involves a group of...
BioFiles 2007, 2.4, 7.
Keywords: Absorption, Cancer, Clinical, Cloning, Combinatorial synthesis, Diseases, Enzymology, Gene expression, Genetic, Immunology, Pharmaceutical, Reductions, Transfection

Live Cell Cycle Assay

Regulation of the cell cycle involves processes crucial to the survival of a cell, including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division associa...
Kevin Su1, Nick Asbrock1, Vi Chu1, Tomas Kraus2
1MilliporeSigma, Temecula, CA; 2Institute of Organic Chemistry and Biochemistry AS CR, Czech Republic
Keywords: Building blocks, Cancer, Cell culture, Cell division, Cell proliferation, Centrifugation, Confocal microscopy, Culture media, Enzyme-linked immunosorbent assay, Flow cytometry, Genetic, Microscopy, Peptide synthesis

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?