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  • B2261 - bisBenzimide H 33342 trihydrochloride

B2261 Sigma-Aldrich

bisBenzimide H 33342 trihydrochloride

≥98% (HPLC and TLC)

Synonym: 2′-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, bisBenzimide

  • CAS Number 23491-52-3

  • Empirical Formula (Hill Notation) C27H28N6O · 3HCl · xH2O

  • Molecular Weight 561.93 (anhydrous basis)

  •  Beilstein/REAXYS Number 1234011

  •  MDL number MFCD00012678

  •  PubChem Substance ID 24891639



Related Categories Alphabetical, Apoptosis Detection, Apoptosis and Cell Cycle, B, Biochemicals and Reagents,
Quality Level   PREMIUM
assay   ≥98% (HPLC and TLC)
solubility   H2O: 20 mg/mL
  phosphate buffer: precipitates
suitability   suitable for fluorescence
storage temp.   −20°C



1 g in glass bottle

100, 500 mg in glass bottle

25 mg in poly bottle


Useful for staining DNA, chromosomes and nuclei. May be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

bisBenzimide H 33342 trihydrochloride has been used for the staining of viable and apoptotic cells. It has been used for the staining of nuclei in cells, which were analyzed by flow cytometry.

Biochem/physiol Actions

Membrane-permeable, fluorescent DNA stains with low cytotoxicity that intercalate in A-T regions of DNA.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Certificate of Origin


Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is a typical concentration of Product B2261, bisBenzimide H 33342 trihydrochloride,for staining DNA in cells?
200 micrograms per mL.
Can solvents other than water be used to dissolve Product B2261, bisBenzimide H 33342 trihydrochloride?
Hoechst 33342 can be dissolved in N,N-dimethylformamide (DMF).
Which Hoechst dye is suitable for chromosomal analysis?
Hoechst 33258 (Product No. B2883) is the preferred Hoechst dye for chromosomal analysis. Hoechst 33342 (Product No. B2261) is a poor fluorochrome for chromosomal analysis.
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