B3051 Millipore

Brucella broth base

for microbiology

  •  NACRES NA.85



Related Categories A - M, All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Liquid Media/Broths, Brucella,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
application(s)   microbiological culture: suitable
  pathogen testing: suitable
  sample preparation: suitable
pH   7.0±0.2 (25 °C)
Featured Industry   Clinical Testing
Food and Beverages
suitability   selective for Brucella spp.
  selective for Campylobacter spp.



Recommended for enrichment, isolation and cultivation of Brucella species.


Components (g/L):
Casein Enzymic Hydrolysate 10.00
Peptic Digest of Animal Tissue 10.00
Yeast Extract 2.00
Dextrose 1.00
Sodium Chloride 5.00
Sodium bisulphite 0.10

Preparation Note

Supplements :
50 ml/l heat inactivated (H1138) horse serum
2 vial/l Campylobacter Growth Supplement (Cat. No. C2847)
50-70 ml/l defibrinated sheep blood
5′000 IU/l polymyxin B (Cat. No. 81334)
25′000 IU/l bacitracin (Cat. No. 11702)
100′000 IU/l nystatin Cat. No. 74721)
100 mg/l cycloheximide (Cat. No. 01810)

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Campylobacter Detection: Rapid Testing Solutions for the Detection of Campylobacter

Campylobacter spp. are regarded as the leading cause of bacterial gastroenteritis in humans worldwide. Infections are usually caused by consuming cross-contaminated or insufficiently processed food (...
Keywords: Adhesion, Aerobic, Antibiotics, Clinical, Culture media, Gastrointestinal, Gene expression, Homogenization, Microbiology, Polymerase chain reaction

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Peer-Reviewed Papers


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H1138 Horse Serum, Donor Herd, USA origin, Heat inactivated, sterile-filtered, suitable for cell culture

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