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B4380 Sigma-Aldrich

Bromobimane

≥97%

Synonym: Monobromobimane

  • CAS Number 71418-44-5

  • Empirical Formula (Hill Notation) C10H11BrN2O2

  • Molecular Weight 271.11

  •  Beilstein/REAXYS Number 4430959

  •  MDL number MFCD00036951

  •  PubChem Substance ID 24891769

  •  NACRES NA.47

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Properties

Related Categories B, Biochemicals and Reagents, Cell Biology, Fluorescence, Fluorescent Labeling of Sulfhydryls,
Quality Level   200
assay   ≥97%
form   powder
application(s)   hematology: suitable
  histology: suitable
mp   161 °C
solubility   acetonitrile: 20 mg/mL
ε (extinction coefficient)   4.6-5.1 at 396-398 nm in H2O
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   −20°C
SMILES string   CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O
InChI   1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3
InChI key   AHEWZZJEDQVLOP-UHFFFAOYSA-N

Description

Application

Bromobimane has been used for the quantitative measurement of free hydrogen sulfide in vivo and in vitro. It has been used for the labeling of proteins containing thiol groups.

Packaging

25, 100 mg in poly bottle

Biochem/physiol Actions

Bromobimane is a nonfluorescent molecule which can react with small thiols and reactive protein thiol groups. The reaction with thiols converts the nonfluorescent bromobimane into a water-soluble fluorescent molecule.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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