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B8647 Sigma-Aldrich

Brilliant Blue R Concentrate

suitable for SDS-PAGE, methanol solution

Synonym: Acid Blue 83, Brilliant Blue R, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

  • CAS Number 6104-59-2

  • Empirical Formula (Hill Notation) C45H44N3NaO7S2

  • Molecular Weight 825.97

  •  Colour Index Number 42660

  •  Beilstein/REAXYS Number 5718025

  •  MDL number MFCD00041762

  •  PubChem Substance ID 24892085

  •  NACRES NA.47

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Properties

Related Categories B, Cell Biology, Coomassie Stains, Core Bioreagents, Hematology and Histology,
Quality Level   200
form   methanol solution
application(s)   hematology: suitable
  histology: suitable
suitability   suitable for SDS-PAGE
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1
InChI   1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1
InChI key   NKLPQNGYXWVELD-UHFFFAOYSA-M

Description

Packaging

1 ea in poly bottle

Application

Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining.
Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).

For detection of protein bands following electrophoresis.

Reconstitution

After dilution with deionized water to a final volume of one liter, solution will contain 0.25% (w/v) Brilliant Blue R, 40% (v/v) methanol and 7% (v/v) acetic acid.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Target organs 
Eyes
RIDADR 
UN1230 - class 3 - PG 2 - Methanol, solution
WGK Germany 
WGK 3
Flash Point(F) 
57.2 °F - closed cup - Not applicable
Flash Point(C) 
14 °C - closed cup - Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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