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C0537 Sigma-Aldrich

5-Carboxyfluorescein

99% (HPLC)

Synonym: 5-FAM

  • CAS Number 76823-03-5

  • Empirical Formula (Hill Notation) C21H12O7

  • Molecular Weight 376.32

  •  Beilstein/REAXYS Number 57037

  •  MDL number MFCD00036874

  •  PubChem Substance ID 24892258

  •  NACRES NA.47

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Properties

Related Categories C, Cell Biology, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
Quality Level   200
assay   99% (HPLC)
form   powder
application(s)   hematology: suitable
  histology: suitable
solubility   0.1 M NaOH: 9.80-10.20 mg/mL, clear to slightly hazy
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   2-8°C
SMILES string   OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35
InChI   1S/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)
InChI key   NJYVEMPWNAYQQN-UHFFFAOYSA-N

Description

Application

5-Carboxyfluorescein has been used as an internal marker in capillary isotachophoresis to quantify the charge-based low density lipoprotein subfractions.

Packaging

50 mg in poly bottle

Biochem/physiol Actions

5-Carboxyfluorescein is used for the staining of cells to differentiate between live and dead cells. It helps in monitoring the integrity and intactness of the cell membrane. It is also used for the measurement of intracellular pH. 5-Carboxyfluorescein diacetate is a non-fluorescent molecule which is cleaved by intracellular esterases, thereby giving a green fluorescent product 5-carboxyfluorescein. In viable cells, the fluorescent molecule is impermeable to the cellular membrane.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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