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C3023 Sigma-Aldrich

CHAPS hydrate

≥98% (HPLC)

Synonym: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS

  • CAS Number 331717-45-4

  • Empirical Formula (Hill Notation) C32H58N2O7S · xH2O

  • Molecular Weight 614.88 (anhydrous basis)

  •  PubChem Substance ID 24892560

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Description

General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

0.2 w/v of CHAPS has been used to remove cerebral endothelium by brain perfusion. 2% (w/v) CHAPS has been used as rehydration buffer along with Thiourea and Urea in protein extraction. 10 mM CHAPS has been used along with 0.15 M NaCl, Tris–Cl, pH 7.6, EDTA as lysis buffer in Western Blot analysis. CHAPS has been used as hybridization buffer in in-situ hybridization method of immature mammalian CNS.

Packaging

1, 5, 25, 100 g in poly bottle

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Certificate of Origin

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Protocols

Solubilization of Membrane Proteins

Extracted from Purifying Challenging Proteins - Principles and Methods, GE Healthcare, 2007
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Crystallization, Degradations, Detergents, Dot blot, Enzyme activity, Filtration, Gene expression, Indicators, Ion Exchange, Ligands, PAGE, Purification, Separation, Solvents, Western blot

Peer-Reviewed Papers
15

References

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