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C5070 Sigma-Aldrich

CHAPS hydrate

BioXtra, ≥98% (TLC)

Synonym: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS

  • CAS Number 331717-45-4

  • Empirical Formula (Hill Notation) C32H58N2O7S · xH2O

  • Molecular Weight 614.88 (anhydrous basis)

  •  PubChem Substance ID 24892750

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Core Bioreagents, Detergents,
Quality Level   ELITE
description   zwitterionic
InChI Key   SJCUTFKCLFLIFE-JWTJKVBLSA-N
product line   BioXtra
assay   ≥98% (TLC)
mol wt   614.88 g/mol (anhydrous basis)
application(s)   protein purification: suitable
  protein quantification: suitable
impurities   ≤0.001% Phosphorus (P)
  ≤0.1% Insoluble matter
ign. residue   ≤0.1%
mp   157 °C (dec.) (lit.)
solubility   H2O: 0.1 M, clear, colorless
anion traces   chloride (Cl-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Na: ≤0.005%
  Pb: ≤0.001%
  Zn: ≤0.0005%

Description

General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

• 10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
• 0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
• HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.

Packaging

1, 5 g in poly bottle

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Certificate of Origin

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Single- & multi-channel pipettes from BRAND
Protocols & Articles
Peer-Reviewed Papers
15

References

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