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D1435 Sigma-Aldrich

Dimethyl sulfoxide

meets EP testing specifications, meets USP testing specifications

Synonym: DMSO

  • CAS Number 67-68-5

  • Linear Formula (CH3)2SO

  • Molecular Weight 78.13

  •  Beilstein/REAXYS Number 506008

  •  EC Number 200-664-3

  •  MDL number MFCD00002089

  •  PubChem Substance ID 57654071

  •  NACRES NA.71

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Properties

Related Categories Cell Culture, Dimethyl Sulfoxide (DMSO), Hepatocytes, Primary Human Cell Culture, Solvent by Type,
Agency/Method   meets EP testing specifications
  meets USP testing specifications
Quality Level   200
vapor density   2.7 (vs air)
vapor pressure   0.42 mmHg ( 20 °C)
assay   ≥99.5%
autoignition temp.   573 °F
expl. lim.   42 %, 63 °F
application(s)   cryopreservation: suitable
impurities   ≤0.1% water (Karl Fischer, USP)
  ≤0.2% water (Karl Fischer, EP)
color   colorless
refractive index   n20/D 1.478-1.479
  n20/D 1.479 (lit.)
bp   189 °C (lit.)
mp   16-19 °C (lit.)
solubility   H2O: miscible (completely)
density   1.100-1.104 g/mL at 25 °C (relative density (EP))
  1.10 g/mL (lit.)
UV absorption   λ: 285 nm Amax: ≤0.20
  λ: 295 nm Amax: ≤0.20
SMILES string   CS(C)=O
InChI   1S/C2H6OS/c1-4(2)3/h1-2H3
InChI key   IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Description

Application

Dimethyl sulfoxide has been used:
• for the dissolution of tetramethylrhodamine dye, which is used as fluorophore for fluorescence in- situ hybridization (FISH) experiments
• as a control in indirect immunofluorescent assay (IFA) of swine testicular (ST) cells
• for the suspension and separation of buccal cells
• a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs.

Packaging

1 L in glass bottle

500 mL in glass bottle

Biochem/physiol Actions

Dimethyl sulfoxide (DMSO) is a low molecular weight, less hydrophilic and a freely permeable cryoprotectant. It is a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant and vitrification agent for the preservation of cells, tissues and organs.

Caution

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

Other Notes

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Safety & Documentation

Safety Information

RIDADR 
NA 1993 / PGIII
WGK Germany 
WGK 1
RTECS 
PV6210000
Flash Point(F) 
188.6 °F - closed cup
Flash Point(C) 
87 °C - closed cup

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

What is the molarity of DMSO?
DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.
What is the DMSO dissolved in?
DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.
Where does DMSO come from?
DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.
Does DMSO have any synthetic utility?
DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
Dimethyl sulfoxide (DMSO) should be a liquid. Why did it arrive as a solid?
DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.
What is the difference between Dimethyl sulfoxide (DMSO) Products. D1435 and D2438?
Products D1435 and D2438both meet the USP specifications. The D2438 is sterile-filtered, but D1435 is not. Furthermore, the D2438 is quality control tested for endotoxin and use-tested for cell freezing.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Is Dimethyl sulfoxide a sterile product?
No, this product is not considered sterile.Product Nos. D2560 and D2438 are sterile-filtered.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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Description

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D2650 Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

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