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E1769 Sigma-Aldrich

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride

BioXtra

Synonym: N-Ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride, EDAC, EDC hydrochloride, EDC, WSC hydrochloride

  • CAS Number 25952-53-8

  • Empirical Formula (Hill Notation) C8H17N3 · HCl

  • Molecular Weight 191.70

  •  Beilstein/REAXYS Number 5764110

  •  EC Number 247-361-2

  •  MDL number MFCD00012503

  •  PubChem Substance ID 24894433

  •  NACRES NA.25

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Properties

Related Categories Crosslinking, Heterobifunctional Cross-Linking Reagents, Molecular Biology, Protein Modification, Protein Structural Analysis,
Quality Level   200
product line   BioXtra
impurities   ≤0.005% Phosphorus (P)
  ≤0.1% Insoluble matter
ign. residue   ≤0.1%
mp   110-115 °C (lit.)
solubility   H2O: 0.5 M, clear to very slightly hazy, colorless to very faintly yellow
anion traces   sulfate (SO42-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Na: ≤0.005%
  Pb: ≤0.001%
  Zn: ≤0.0005%
storage temp.   −20°C
SMILES string   Cl.CCN=C=NCCCN(C)C
InChI   1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
InChI key   FPQQSJJWHUJYPU-UHFFFAOYSA-N

Description

Application

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride has been used:
• for post-fixation and crosslinking of small ribonucleic acid (RNAs) species from formalin fixed paraffin embedded (FFPE) tissues, for fluorescent in situ hybridisation (FISH) and immunofluorescence (IF) signals
• for cross-linking of nanofiber matrices with mouse lung extract
• for activation of substrate XNA oligonucleotides

Packaging

1, 5, 10, 25 g in poly bottle

Biochem/physiol Actions

Water soluble condensing reagent. EDAC is generally utilized as a carboxyl activating agent for amide bonding with primary amines. In addition, it will react with phosphate groups. EDAC has been used in peptide synthesis; crosslinking proteins to nucleic acids; and preparation of immunoconjugates as examples. Typically, EDAC is utilized in the pH range 4.0-6.0 without buffers. In particular, amine and carboxylate buffers should be avoided.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
FF2200000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Poly(N-isopropylacrylamide)-based Smart Surfaces for Cell Sheet Tissue Engineering

Tissue engineering has become a key therapeutic tool in the treatment of damaged or diseased organs and tissues, such as blood vessels and urinary bladders.1 Nonetheless, major challenges still need ...
Masamichi NakayamaTeruo Okano and Françoise M. Winnik
Material Matters 2010, 5.3, 56.
Keywords: Adhesion, Biomaterials, Cell culture, Clinical, Dehydration reaction, Growth factors, Hydration reaction, Immobilization, Inflammation, Ligands, Magnetic resonance spectroscopy, Polymerization reactions, Radical polymerization, Rearrangements, Reversible addition-fragmentation chain transfer polymerizations, Solvents

Protocols

EDC-mediated couplings of Polyester-16-hydroxyl-1-carboxyl bis-MPA dendron

This monodisperse compound has multiple surface hydroxyl groups and a single carboxylic acid focal point function. The carboxylic acid function is well suited for EDC-mediated coupling to amine-conta...
Keywords: Antibiotics, Biochemistry, Solvents

EDC-mediated couplings of Polyester-32-hydroxyl-1-carboxyl bis-MPA dendron

This monodisperse compound has multiple surface hydroxyl groups and a single carboxylic acid focal point function. The carboxylic acid function is well suited for EDC-mediated coupling to amine-conta...
Keywords: Antibiotics, Biochemistry, Solvents

EDC-mediated couplings of Polyester-8-hydroxyl-1-carboxyl bis-MPA dendron

Theoretical MW: 830.82 g/mol Average Molecular Formula: C35H58O22 Number of hydroxyl groups: 8 Number of carboxylic acid groups: 1
Keywords: Antibiotics, Biochemistry, Solvents

Peer-Reviewed Papers
15

References

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