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E2028 Sigma-Aldrich

Ethidium bromide monoazide

≥95% (HPLC), solid

Synonym: 3-Amino-8-azido-5-ethyl-6-phenylphenanthridinium bromide, Ethidium monoazide bromide

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Properties

Related Categories Cell Biology, Chemical Biology, Chemical Synthesis, Conjugation Chemistry: Azides, Alkynes and Other Reagents, Copper Click Chemistry,
Quality Level   100
assay   ≥95% (HPLC)
form   solid
application(s)   hematology: suitable
  histology: suitable
color   orange
solubility   DMF: soluble
  ethanol: soluble
ε (extinction coefficient)   ≥5200 at 459-465 nm
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   −20°C
SMILES string   [Br-].CC[n+]1c(-c2ccccc2)c3cc(ccc3c4ccc(N)cc14)N=[N+]=[N-]
InChI   1S/C21H17N5.BrH/c1-2-26-20-12-15(22)8-10-18(20)17-11-9-16(24-25-23)13-19(17)21(26)14-6-4-3-5-7-14;/h3-13,22H,2H2,1H3;1H
InChI key   GHUXAYLZEGLXDA-UHFFFAOYSA-N

Description

Packaging

5 mg in glass bottle

Biochem/physiol Actions

Photoactive stain covalently binds to nucleic acids in solution and in cells with damaged membranes.

Used to footprint drug binding sites on DNA, detect non-viable cells, identify ethidium binding sites on DNA and tRNA, and selectively inactivate gene expression.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
SF7941850
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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