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E4382 Sigma-Aldrich

Eosin Y disodium salt

certified by the Biological Stain Commission

Synonym: 2′,4′,5′,7′-Tetrabromofluorescein disodium salt, Acid Red 87, Bromo acid J. TS, XL, or XX, Bromofluorescein, Bronze Bromo ES, D&C;Red No. 22, Eosin yellowish

  • CAS Number 17372-87-1

  • Empirical Formula (Hill Notation) C20H6Br4Na2O5

  • Molecular Weight 691.85

  •  Colour Index Number 45380

  •  Beilstein/REAXYS Number 3586809

  •  EC Number 241-409-6

  •  MDL number MFCD00005040

  •  PubChem Substance ID 24894551

  •  NACRES NA.47

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Properties

Related Categories Biochemicals and Reagents, Cell Biology, Certified Stains & Dyes, Core Bioreagents, E-F,
Quality Level   200
grade   certified by the Biological Stain Commission
form   powder
composition   Dye content, ≥90%
application(s)   hematology: suitable
  histology: suitable
solubility   H2O: 1 mg/mL
density   1.018 g/cm3
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Na+].[Na+].[O-]C(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24
InChI   1S/C20H8Br4O5.2Na/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;;/h1-6,25H,(H,27,28);;/q;2*+1/p-2
InChI key   SEACYXSIPDVVMV-UHFFFAOYSA-L

Description

Application

Eosin Y disodium salt has been used as a counterstain in immunocytochemistry and immunohistochemistry.

Packaging

25, 100 g in glass bottle

Biochem/physiol Actions

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Suitability

Certified for use as a counterstain against hematoxylin; as a constituent of Wright stain; in modified Nocht′s stain; as a constituent in eosin-methylene blue medium.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
LM5850000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles
Peer-Reviewed Papers
15

References

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