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E9884 Sigma-Aldrich

Ethylenediaminetetraacetic acid

ACS reagent, 99.4-100.6%, powder

Synonym: (Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid

  • CAS Number 60-00-4

  • Linear Formula (HO2CCH2)2NCH2CH2N(CH2CO2H)2

  • Molecular Weight 292.24

  •  Beilstein/REAXYS Number 1716295

  •  EC Number 200-449-4

  •  MDL number MFCD00003541

  •  eCl@ss 39030907

  •  PubChem Substance ID 24894705

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Properties

Related Categories Biochemicals and Reagents, Chelation/Complexation Compounds, Chelators, Chemical Synthesis, Core Bioreagents,
grade   ACS reagent
  ACS reagent
InChI Key   KCXVZYZYPLLWCC-UHFFFAOYSA-N
assay   99.4-100.6%
form   powder
impurities   ≤0.005% Insol. dil. NH4OH
  ≤0.1% Nitrilotriacetic acid ([HOCOCH2]3N)
ign. residue   ≤0.2%
mp   250 °C (dec.) (lit.)
solubility   water: soluble 0.4 g/L at 20 °C
cation traces   Ca: ≤0.001%
  Fe: ≤0.005%
  Mg: ≤5 ppm
  heavy metals (as Pb): ≤0.001%

Description

General description

Ethylenediaminetetraacetic acid (EDTA) is a hydrophilic metal chelating agent that transforms metal ions into inactive, cyclic metal complexes. Hence, it has industrial application for resolving metal contamination.

Application

Ethylenediaminetetraacetic acid has been used:
• as a component of labeling buffer, which is used in the washing step during cell fractionation{155)
• for investigating the effect of papain enzyme on wound debridement and other skin issues
• for harvesting rat arterial smooth muscle cell after its pre-treatment for its use in flow cytometry analysis

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is not easily administered by the gastrointestinal tract. Intravenous administration of sodium EDTA results in hypocalcemic tetany. It is generally useful in plasma or molecular diagnostics and acts by chelating calcium. EDTA serves as an anticoagulant for haematological cell counting and cell morphological analysis.

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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
2
RTECS 
AH4025000

Documents

Certificate of Analysis

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Certificate of Origin

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cancer, Cell attachment, Cell culture, Cryopreservation, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

Peer-Reviewed Papers
15

References

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