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ED2SS Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

reagent grade, 98.5-101.5% (titration)

Synonym: Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

  • CAS Number 6381-92-6

  • Empirical Formula (Hill Notation) C10H14N2Na2O8 · 2H2O

  • Molecular Weight 372.24

  •  Beilstein/REAXYS Number 3900609

  •  EC Number 205-358-3

  •  PubChem Substance ID 24894708

  •  NACRES NA.77



Related Categories Biochemicals and Reagents, Broad Spectrum Inhibitors of Proteolytic Enzyme Classes, Calcium Signaling Research Tools, Carboxypeptidase A, Carboxypeptidase B,
Quality Level   200
biological source   synthetic (organic)
grade   reagent grade
assay   98.5-101.5% (titration)
form   powder
mp   248 °C (dec.) (lit.)
solubility   H2O: soluble, clear, colorless
storage temp.   room temp
SMILES string   O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
InChI   1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2


General description

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid disodium salt (EDTA) is a blood anticoagulant and contributes to the pathogenesis of pseudothrombocytopenia. It chelates with calcium in the blood and inhibits clotting and is routinely used in haematological tests. It enhances the antibacterial activity of lysozyme. EDTA used in chelation therapy chelates with calcium and favors dilation of artery, solubilisation of atheromatous plaques in atherosclerotic vascular disease. EDTA chelation therapy protects from lipid peroxidation events in liver fibrosis.


Ethylenediaminetetraacetic acid disodium salt dihydrate has been used as a component of the plasma membrane resuspension buffer, inhibitor′s preservative solution (IS) and luciferase activity assay mix.

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.


1 kg in poly bottle

2.5, 5 kg in poly drum

50, 100, 250, 500 g in poly bottle

Biochem/physiol Actions

Zinc-dependent metalloproteinases, as well as other proteases that are stabilized by calcium, can be effectively inhibited by chelation of divalent metal ions with EDTA. Other chelators such as EGTA, specific for calcium, and 1,10-phenanthroline, highly specific for zinc, can be used to target these two different types of proteases.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Target organs 
Respiratory Tract
Personal Protective Equipment 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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pHast Pack
Protocols & Articles


Enzymatic Assay and ATP Sensitivity Test of Luciferase

To standardize a procedure to determine the enzymatic activity of Luciferase and/or to determine the ATP detection limit of Luciferase.
Keywords: Biochemistry, Enzyme activity, Enzymology, Size-exclusion chromatography

Enzymatic Assay of Choloylglycine Hydrolase

To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
Keywords: Enzyme activity

Enzymatic Assay of Superoxide Dismutase

This procedure applies to all products that have a specification for superoxide dismutase activity by enzymatic determination.
Keywords: Catalysis, Reductions

Peer-Reviewed Papers


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