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EDS Sigma-Aldrich

Ethylenediaminetetraacetic acid

BioUltra, anhydrous, ≥99% (titration)

Synonym: (Ethylenedinitrilo)tetraacetic acid, EDTA, Edathamil, Ethylenedinitrilotetraacetic acid

  • CAS Number 60-00-4

  • Linear Formula (HO2CCH2)2NCH2CH2N(CH2CO2H)2

  • Molecular Weight 292.24

  •  Beilstein Registry Number 1716295

  •  EC Number 200-449-4

  •  MDL number MFCD00003541

  •  eCl@ss 39030907

  •  PubChem Substance ID 24894713




1 kg in poly bottle

100, 500 g in poly bottle


EDTA has been used:
• as a medium supplement in embryo culture using synthetic oviduct fluid culture 1 (SOFC1) medium
• in incubation of EpCAM+ cells with fluorescein isothiocyanate (FITC) conjugated antibody for cell sorting analysis
• in pre-treatment of single cell suspension of dendritic cells from spleen for cell sorting with magnetic beads

General description

Ethylenediaminetetraacetic acid (EDTA) is a substituted diamine and a versatile compound in household with industrial applications. EDTA chelates metal divalent cations like calcium, magnesium, zinc, copper, manganese by forming metal-EDTA complexes. It finds its application in cleaning products and detergent formulations. In paper and pulp industries, EDTA reduces the adverse effect of metal ions on bleaching. It functions as an antibacterial agent by removing calcium and magnesium divalent cations from the outer membrane and causes loss of lipopolysaccharide from the membrane, making the bacteria susceptible to bactericidal agents. Free EDTA has adverse effect on reproduction and development in mammals. EDTA can chelate ions from deposits that contribute to atherosclerosis, cancers and heart diseases. It can help to overcome cell membrane injury by reducing radical reactions and oxidation processes. It binds to calcium and reduces the risk of hypercalcinemia in cancer patients. Transmembrane ammonium EDTA gradient method aids in drug delivery, increasing drug retention and efficacy of therapeutics and lowers the cytotoxicity levels.

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Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
UN 3077 9 / PGIII
WGK Germany 


Certificate of Analysis

Certificate of Origin

High Quality GMP-Buffers

Protein Stabilizer
Protocols & Articles

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