G1522 Sigma-Aldrich

Gentamicin solution

Hybri-Max, 50 mg/mL gentamicin, sterile-filtered, BioReagent, suitable for hybridoma

Synonym: Garamycin, Gentiomycin C



Related Categories A - K, Aminoglycosides, Antibacterial, Antibiotic Solutions, Antibiotics,
grade   Hybri-Max
sterility   sterile-filtered
product line   BioReagent
form   liquid
quality   Prepared in tissue culture grade water.
concentration   50 mg/mL gentamicin
application(s)   cell culture | hybridoma: suitable
impurities   endotoxin, tested
color   colorless to light yellow
density   1.000 g/cm3
suitability   suitable for hybridoma
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
storage temp.   2-8°C



Gentamicin is an aminoglycoside complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is used as the sulfate salt. There are three components, each consisting of five basic nitrogens and requiresing five equivalents of sulfuric acid per mole of gentamicin base.


Gentamicin sulfate is a broad-spectrum antibiotic used as a selection agent (gentamicin-resistance gene) in molecular biology and cell culture applications. This product is formulated to contain 50 mg/mL Gentamicin in deionized water and is recommended for use in eukaryotic cell culture at a volume of 1 milliliter per liter.

Biochem/physiol Actions

Mode of Action: Gentamicin inhibits protein synthesis by binding to the 30S subunit of the ribosome.

Antimicrobial spectrum: Includes Gram-negative and Gram-positive bacteria, including strains resistant to tetracycline, chloramphenicol, kanamycin and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus and Streptococcus.


The product is stable at 37° for 5 days and is stored at 2-8°C.

General description

Chemical structure: aminoglycoside

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Certificate of Origin

Request a Serum Sample

Cell Culture Guide
Protocols & Articles


Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

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Antibiotics Selection Guide

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Peer-Reviewed Papers


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G1397 Gentamicin solution, 50 mg/mL in deionized water, liquid, sterile-filtered, BioReagent, suitable for cell culture

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