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G6639 Sigma-Aldrich

Guanidine thiocyanate

≥97% (titration)

Synonym: Guanidinium rhodanide, Guanidinium thiocyanate

  • CAS Number 593-84-0

  • Linear Formula NH2C(=NH)NH2 · HSCN

  • Molecular Weight 118.16

  •  Beilstein/REAXYS Number 3563461

  •  EC Number 209-812-1

  •  MDL number MFCD00013027

  •  PubChem Substance ID 24895264

  •  NACRES NA.55

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Properties

Related Categories Biochemicals and Reagents, Chaotropic Agents, Core Bioreagents, Denaturation, Nucleosides and Nucleotides,
Quality Level   200
biological source   synthetic
assay   ≥97% (titration)
form   powder or crystals
application(s)   RNA extraction: suitable
color   white to off-white
mp   120-122 °C (lit.)
solubility   water: 4 M, clear, colorless
anion traces   chloride (Cl-): ≤2.5%
SMILES string   SC#N.NC(N)=N
InChI   1S/CH5N3.CHNS/c2-1(3)4;2-1-3/h(H5,2,3,4);3H
InChI key   ZJYYHGLJYGJLLN-UHFFFAOYSA-N

Description

Application

Guanidine thiocyanate has been used:
• as a denaturing agent to study its effects on surface activity of globular protein
• for the extraction of total RNA from primary hepatocytes
• as a component of the incubating buffer for extraction of DNA from human bone
• as a component of the guanidine thiocyanate (GITC) lysis buffer for extraction of elephant shark DNA
• as a component of lysis solution for extraction of RNA from rat pancreatic tissues

Chaotropic agent and strong denaturant; solubilizes cells.

Packaging

1 kg in glass bottle

500 g in glass bottle

25, 100, 250 g in poly bottle

Biochem/physiol Actions

Guanidine thiocyanate (GTC) is a powerful protein denaturant and can denature endogenous ribonucleases. It plays a role in separation of ribosomal ribonucleic acid (rRNA) from ribosomes. GTC in combination with phenol and chloroform can be used in isolation of total RNA from cells and tissues.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Supplemental Hazard Statements 
EUH032
Personal Protective Equipment 
RIDADR 
UN 1759 8 / PGIII
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles
Peer-Reviewed Papers
15

References

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