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G9012 Sigma-Aldrich

Glycerol

≥99.5%

Synonym: 1,2,3-Propanetriol, Glycerin

  • CAS Number 56-81-5

  • Linear Formula HOCH2CH(OH)CH2OH

  • Molecular Weight 92.09

  •  Beilstein/REAXYS Number 635685

  •  EC Number 200-289-5

  •  MDL number MFCD00004722

  •  PubChem Substance ID 24895360

  •  NACRES NA.21

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Properties

Related Categories Aliphatic Compounds, Biochemicals and Reagents, Biostabilization Agents, Carbohydrates, Carbohydrates A to Z,
Quality Level   200
vapor density   3.1 (vs air)
vapor pressure   <1 mmHg ( 20 °C)
assay   ≥99.5%
autoignition temp.   698 °F
impurities   ≤0.10% Water (Karl Fischer)
refractive index   n20/D 1.474 (lit.)
bp   182 °C/20 mmHg (lit.)
mp   20 °C (lit.)
density   1.25 g/mL (lit.)
cation traces   heavy metals (as Pb): ≤5 ppm
λ   1 cm path
UV absorption   λ: 205 nm Amax: 1.0
  λ: 225 nm Amax: 0.10
  λ: 280 nm Amax: 0.05
  λ: 320 nm Amax: 0.02
  λ: 340 nm Amax: 0.01
  λ: 400 nm Amax: 0.01
suitability   suitable for component for culture media
SMILES string   OCC(O)CO
InChI   1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2
InChI key   PEDCQBHIVMGVHV-UHFFFAOYSA-N

Description

General description

Glycerol is odourless, colorless, viscous in nature, and exists as a sweet tasting liquid. It can be derived naturally as well as from petrochemical feedstock. Glycerol has a wide variety of applications, and is one of the most valuable and versatile chemical substances in nature. It can be used as an emollient, solvent, sweetening agent, in pharmaceutical formulations, cosmetics, foodstuffs and toiletries. It is very stable and can be easily stored under normal temperature; also it is non-irritating and has no adverse impact to the environment.

Application

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel′s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Packaging

1 L in poly bottle

100, 500 mL in poly bottle

Biochem/physiol Actions

Glycerol is hygroscopic in nature and is soluble in water owing to its three hydrophilic alcoholic hydroxyl groups. It can form both inter- and intramolecular hydrogen bonds, making it a very flexible molecule. The physiologic effect of glycerine is due to cell-mediated immunity, increased IgG production and increased histamine release.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
MA8050000
Flash Point(F) 
390.2 °F - Pensky-Martens closed cup
Flash Point(C) 
199 °C - Pensky-Martens closed cup

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the viscosity of glycerol?
The viscosity of glycerol is 648 Centistokes at 20 Deg C (68 Deg F).
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Protocols & Articles

Protocols

Enzymatic Assay of Chitosanase from Streptomyces Species

This procedure applies to Sigma-Aldrich Product Number, C0794. This procedure is NOT to be used to assay Chitosanase from Streptomyces griseus, Sigma-Aldrich Product Number, C9830.
Keywords: Biochemistry, Enzyme activity, Microbiology

Immunoprecipitation Procedure

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Buffers, Cell disruption, Centrifugation, Enzyme activity, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, PAGE, Size-exclusion chromatography, Western blot

Peer-Reviewed Papers
15

References

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