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H3136 Sigma-Aldrich

Hematoxylin

certified by the Biological Stain Commission

Synonym: Natural Black 1

  • CAS Number 517-28-2

  • Empirical Formula (Hill Notation) C16H14O6 · xH2O

  • Molecular Weight 302.28 (anhydrous basis)

  •  Colour Index Number 75290

  •  Beilstein Registry Number 91400

  •  EC Number 208-237-3

  •  MDL number MFCD00078111

  •  PubChem Substance ID 24895554

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Properties

Related Categories Cell Biology, Certified Stains & Dyes, Core Bioreagents, G-H-I, Hematology and Histology,
grade   certified by the Biological Stain Commission
InChI Key   WZUVPPKBWHMQCE-XJKSGUPXSA-N
form   powder
mp   200 °C (dec.) (lit.)
solubility   ethanol: 1 mg/mL
Gene Information   rat ... Adora1(29290), Adora2a(25369)

Description

Packaging

25, 100 g in glass bottle

Application

Hematoxylin has been used:
• to visualize anatomical features and to detect nucleus locus coeruleus adjacent to the periaqueductal gray region in brainstem samples
• as a nuclear counterstain to visualize the nucleus, adipocytes were counterstained with a hematoxylin reagent
• to stain lung tissue for histological analysis

General description

Hematoxylin is a basophilic dye extracted from the tree Heamatoxylum campechianum. It specifically binds to acidic compounds and thus is useful for staining cell nuclei which contain acidic substances: DNA and RNA. Hematoxylin, when oxidized forms hematein, which has thick pale blue purple colour. Hematoxylin is often used along with eosin, in histological study under light microscope.

Suitability

Certified for use in Weigert′s iron hematoxylin, Mayer′s hemalum, Harris hemalum and in Heidenhain′s technique

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
MH7875000

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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