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H3149 Sigma-Aldrich

Heparin sodium salt from porcine intestinal mucosa

Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture



Related Categories Biochemicals and Reagents, Carbohydrates, Coagulation Proteins and Reagents, Core Bioreagents, General Reagents,
type   Grade I-A
product line   BioReagent
form   powder
solubility   H2O: 50 mg/mL
suitability   suitable for cell culture



10000, 25000, 50000, 100000, 250000, 500000, 1000000 units in poly bottle


• Heparin sodium salt from porcine intestinal mucosa has been used in sLRP (soluble circulating low density lipoprotein receptor-related protein-1) purification from human plasma.
• It has been used as a supplement in M199 medium for culturing of human umbilical vein endothelial cells.
• It has been used as an anticoagulant agent during isolation of blood.

General description

Heparin is mainly responsible for the delay in the coagulation of blood. It enhances the antithrombin-mediated inactivation of proteases in the coagulation pathway.

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Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

How is Heparin sodium salt from porcine intestinal mucosa, Product H3149, solubilized?
Heparin sodium salt from porcine intestinal mucosa is soluble in deionized water at a concentrationi of 50 mg/mL.  This answer may also be found on the product detail page. http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIAL/H3149
How much of Heparin sodium salt from porcine intestinal mucosa, Product H3149, is used for cell culture?
Solutions at 0.5 mg per mL were tested in cell culture. Start at this concentration, and titer from there to determine the optimal concentration for usage.
How are solutions Heparin sodium salt from porcine intestinal mucosa, Product H3149, stored?
Solutions can be stored at 2-8C for up to 2 years, if sterile filtered through a 0.22 micron filter. Benzyl alcohol (0.06-1%) can be added to solutions to prevent microbial growth. The answer can also be found by clicking: http://www.sigmaaldrich.com/etc/medialib/docs/Sigma-Aldrich/Product_Information_Sheet/h3149pis.Par.0001.File.tmp/h3149pis.pdf
How many milligrams of Heparin sodium salt, Product H3149, are in the bottle?
The potency, in units/mg, is lot-specific; the value can be found on the certificate of analysis. It is typically over 140 units per mg. The number of milligrams can be calculated by dividing the number of units by the potency in units per mg.
What are the units used for Heparin sodium salt?
Units are USP (United States Pharmacopeia units), which is defined by the USP Heparin Sodium Reference Standard.  As of October 2009, the USP aligned the USP units with the International Units. Please see the following link that describes the change in the USP monograph: http://www.usp.org/usp-nf/hot-topics/heparin/official-stage-2-monographs
What is the molecular weight of Heparin sodium salt?
Heparin is a mixture of polyanion chains having molecular weights ranging from 6,000 to 30,000 Daltons, with most chains in the range of 17,000 to 19,000 Daltons.
How much Heparin sodium salt, is used to prevent coagulation?
We suggest using 20 to 50 units per mL of blood.  
What is the best way to sterilize Heparin sodium salt?
Although solutions may be autoclaved at 121 °C for 5 to 10 minutes, we generally recommend sterile filtration. This is because prolonged autoclaving may have an effect on its activity.  
What concentration of Heparin sodium salt, can I use to inhibit RNAse activity?
What concentration can I use to inhibit RNase activity?" Change answer to: "Heparin has been used at concentrations ranging from 100 to 500 µg/mL. This has been reported in the J. Biol. Chem., 248, 2095 (1973).
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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