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I3021 Sigma-Aldrich

IGEPAL® CA-630

viscous liquid

Synonym: Octylphenoxy poly(ethyleneoxy)ethanol, branched

  • CAS Number 9002-93-1

  • Linear Formula (C2H4O)nC14H22O

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Description

General description

IGEPAL® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL® CA-630 has been used:
• As a component of grinding buffer along with lithium dodecyl sulphate, tris-HCl, EDTA, NaCl, sodium deoxycholate, dithiothreitol and thiourea in RNA extraction process
• Along with Tris- HCl finds its application in incubation of Tachyzoites cultured in vero cells
• As a component of nuclear extract buffer D+ containing HEPES, glycerol, KCl and EDTA for gel shift analysis
• As a component of PI buffer along with NaH2PO4 to wash slides in FISH (fluorescence in situ hybridization) procedure

Packaging

50, 100, 500 mL in poly bottle

Other Notes

Nonionic, non-denaturing detergent

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Caution

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Legal Information

IGEPAL is a registered trademark of Rhodia Operations

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
2
Flash Point(F) 
483.8 °F
Flash Point(C) 
251 °C

Documents

Certificate of Analysis

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Certificate of Origin

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Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles

Protocols

Solubilization of Membrane Proteins

Extracted from Purifying Challenging Proteins - Principles and Methods, GE Healthcare, 2007
Keywords: Buffers, Cell disruption, Centrifugation, Chromatography, Crystallization, Degradations, Detergents, Dot blot, Enzyme activity, Filtration, Gene expression, Indicators, Ion Exchange, Ligands, PAGE, Purification, Separation, Solvents, Western blot

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Flow Cytometry Protocol

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Peer-Reviewed Papers
15

References

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