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I6758 Sigma-Aldrich

IPTG

≥99% (TLC), ≤0.1% Dioxane

Synonym: IPTG, Isopropyl β-D-1-thiogalactopyranoside, Isopropyl β-D-thiogalactoside

  • CAS Number 367-93-1

  • Empirical Formula (Hill Notation) C9H18O5S

  • Molecular Weight 238.30

  •  Beilstein/REAXYS Number 4631

  •  EC Number 206-703-0

  •  MDL number MFCD00063273

  •  PubChem Substance ID 24896093

Purchase

Description

Packaging

1, 5, 10 g in glass bottle

Application

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Other Notes

Non-metabolizable galactose analog.

Reconstitution

A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Protocols

Bacterial Transformation Protocols

Transformation is a key process in molecular cloning, by which multiple copies of recombinant DNA molecules are produced. The ability to take up free, extracellular genetic material is the prerequisi...
Keywords: Antibiotics, Cloning, Detergents, Electrophoresis, Gel electrophoresis, Genetic, Mass spectrometry, Peptide synthesis, transformation

Microbial Growth Protocols

From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Keywords: Antibiotics

Peer-Reviewed Papers
15

References

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