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I9274 Supelco

Invertase from baker's yeast (S. cerevisiae)

200-300 units/mg solid

Synonym: β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
Featured Industry   Food and Beverages
General Analytical
foreign activity   α-glucosidase (maltase), phosphoglucomutase, phosphoglucose isomerase ≤0.01%
  6-phosphogluconic dehydrogenase, α-galactosidase, β-galactosidase, and NADH oxidase ≤0.001%
storage temp.   2-8°C

Description

General description

Invertase is encoded by the SUC2 (Saccharomyces cerevisiae S288C) gene. In yeast this enzyme exist in: glycosylated (secreted) and non-glycosylated (intracellular) form. The external invertase is composed of carbohydrate (50%) and 60,000 D subunits.

Application

Invertase from baker′s yeast (S. cerevisiae) has been used:
• in liquid media (YES) and is used to determine the invertase activity
• supplemented to the reaction mixture in the extraction and analysis of sugars and starch
• for sucrose hydrolysis

Used in the production of confectionary foods and artificial honey.

Packaging

100 mg in poly bottle

Biochem/physiol Actions

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

Unit Definition

One unit will hydrolyze 1.0 μmole sucrose to glucose and fructose per minute at pH 4.6 at 25 °C.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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