L5910 Millipore

Lysine medium

for microbiology, NutriSelect Plus

  •  NACRES NA.85



Related Categories A - M, All Liquid Media/Broths, Biochemical Identification Media by Test, Biochemical Identification Media, A to Z, Candida,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
mfr. no.   NutriSelect Plus
application(s)   microbiological culture: suitable
  microbiology: suitable (microbial identification)
final pH   5.0±0.2 (25 °C)
Featured Industry   Food and Beverages
suitability   nonselective for Candida spp.
  nonselective for Pichia spp.
  nonselective for Saccharomyces spp.
  nonselective for Zygosaccharomyces spp.



Used for isolation and enumeration of wild yeasts in pitching yeasts.


Ingredients (g/L)
Dextrose, 44.50
Monopotassium phosphate, 1.78
Magnesium sulphate, 0.89
Calcium chloride, 0.178
Sodium chloride, 0.089
Adenine, 0.00178
DL-Methionine, 0.000891
L-Histidine, 0.000891
DL-Tryptophane, 0.000891
Boric acid, 0.0000089
Zinc sulphate, 0.0000356
Ammonium molybdate, 0.0000178
Manganese sulphate, 0.0000356
Ferrous sulphate, 0.0002225
L-Lysine, 1.00
Inositol, 0.02
Calcium pantothenate, 0.002
Aneurine, 0.0004
Pyridoxine, 0.0004
p-Aminobenzoic acid, 0.0002
Nicotinic acid, 0.0004
Riboflavin, 0.0002
Biotin, 0.000002
Folic acid, 0.000001
Agar, 17.80

Preparation Note

Suspend 6.6 g of Lysine Medium in 100 ml of distilled water containing 0.84 ml of potassium lactate solution ~60% (Cat. No. 60389). Boil to dissolve the medium completely. Cool to 50 °C. Adjust the pH to 5.0 with a 10% solution of Lactic Acid (Cat. No. 69778) and pour into sterile petri plates.NoteThe degree of contamination is expressed as the number of wild yeast cells per million cells of the original inoculum. The number of cells in the inoculum is important as a small number (100-1000) grow to a limited extent while 10′000 brewing yeast cells provide a direct measure of contaminant wild yeasts.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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