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M0178 Millipore

Middlebrook 7H9 Broth Base

for microbiology

  •  NACRES NA.85

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Certificate of Analysis (COA)
Datasheet (PDF)
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Properties

Related Categories A - M, All Liquid Media/Broths, Clinical Media, Media by Application, Media by Microorganism,
Quality Level   200
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
final pH   6.6±0.2 (25 °C)
Featured Industry   Clinical Testing
Food and Beverages
suitability   mycobacteria

Description

General description

Middlebrook 7H9 Broth Base contains many inorganic salts, which support the growth of mycobacteria. Sodium citrate provides citrate ions in the medium, which retain inorganic cations in the solution.

Application

Middlebrook 7H9 Broth Base has been used for the cultivation Mycobacterium bovis bacillus Calmette-Guérin Pasteur strain.

Components

Ingredients (g/L)
Ammonium sulfate, 0.50
Disodium phosphate, 2.50
Monopotassium phosphate, 1.00
Sodium citrate, 0.10
Magnesium sulfate, 0.05
Calcium chloride, 0.0005
Zinc sulfate, 0.001
Copper sulfate, 0.001
Ferric ammonium citrate, 0.04
L-Glutamic acid, 0.50
Pyridoxine, 0.001
Biotin, 0.0005

Preparation Note

Supplements:
4 ml/l glycerol (Cat. No. 49767)
2 vial/l Middlebrook ADC Growth Supplement (Cat. No.M0553) or
1 g/l of Tween® 80 (Cat. No. P8074)
2 vial/l Middlebrook ADC Growth Supplement (Cat. No. M0553)

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Peer-Reviewed Papers
15

References

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M0553 Middlebrook ADC Growth Supplement, for microbiology

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