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M1428 Millipore

MIO Medium

for microbiology, NutriSelect Plus

Synonym: Motility Indole Ornithine Medium

  •  NACRES NA.85

Purchase

Properties

Related Categories A - M, All Liquid Media/Broths, Biochemical Identification Media by Test, Biochemical Identification Media, A to Z, Decarboxylase Test,
Quality Level   100
grade   for microbiology
form   powder
shelf life   limited shelf life, expiry date on the label
mfr. no.   NutriSelect Plus
application(s)   microbiological culture: suitable
  microbiology: suitable (microbial identification)
final pH   6.5±0.2 (25 °C)
Featured Industry   Clinical Testing
Environmental
Food and Beverages
Veterinary
suitability   nonselective and differential for Enterobacter spp.
  nonselective and differential for Klebsiella spp.
  nonselective and differential for Proteus spp.
  nonselective and differential for enterobacteriaceae

Description

Application

Used for the identification of enterobacteriaceae on the basis of motility, indole production and ornithine decarboxylase activity.

Components

Ingredients (g/L)
Casein enzymatic hydrolysate, 10.00
Peptic digest of animal tissue, 10.00
Yeast extract, 3.00
L-Ornithine hydrochloride, 5.00
Dextrose, 1.00
Bromocresol purple, 0.02
Agar, 2.00

Preparation Note

Suspend 31 g of MIO Medium in 1000 ml of distilled water. Boil to dissolve the medium completely. Dispense 5 ml into each test tube. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 15 minutes. Cool the tubes in an upright position.

Legal Information

NutriSelect is a trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the natural environment, microbes have adapted to the habitats most suitable ...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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