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M8010 Sigma-Aldrich

N,N′-Dimethyl-9,9′-biacridinium dinitrate

used as chemiluminescent reagent

Synonym: 9,9′-Bis(N-methylacridinium nitrate), Lucigenin

  • CAS Number 2315-97-1

  • Empirical Formula (Hill Notation) C28H22N4O6

  • Molecular Weight 510.50

  •  Beilstein/REAXYS Number 3901768

  •  EC Number 219-023-4

  •  MDL number MFCD00011925

  •  PubChem Substance ID 24897210

  •  NACRES NA.47

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Properties

Related Categories Biochemicals and Reagents, Carbohydrate Labels, Carbohydrate Labels for Chromatography, Cell Biology, Chelators and Ion Probes,
Quality Level   100
form   powder
application(s)   hematology: suitable
  histology: suitable
mp   245-250 °C
solubility   acetic acid: 10 mg/mL
fluorescence   λex 319 nm; λem 384 nm (pH 9.0)
  λex 360 nm; λem 449 nm (Reaction product)
  λex 368 nm; λem 505 nm in H2O
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [O-][N+]([O-])=O.[O-][N+]([O-])=O.C[n+]1c2ccccc2c(-c3c4ccccc4[n+](C)c5ccccc35)c6ccccc16
InChI   1S/C28H22N2.2NO3/c1-29-23-15-7-3-11-19(23)27(20-12-4-8-16-24(20)29)28-21-13-5-9-17-25(21)30(2)26-18-10-6-14-22(26)28;2*2-1(3)4/h3-18H,1-2H3;;/q+2;2*-1
InChI key   KNJDBYZZKAZQNG-UHFFFAOYSA-N

Description

Application

N,N′-Dimethyl-9,9′-biacridinium dinitrate has been used in chemiluminescence assay to measure reactive oxygen species production and superoxide production.

Reagent used for the generation of chemiluminescence in the presence of a reductant and oxygen; it is employed in numerous chemiluminescent assays. Lucigenin is specific for superoxide anion radical.

Packaging

1, 5 g in glass bottle

500 mg in glass insert

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Applications, Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Methods, Microscopy, Neurotransmission, Sol-gel, Type

Peer-Reviewed Papers
15

References

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