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M9140 Sigma-Aldrich

Methylene blue

certified by the Biological Stain Commission

Synonym: 3,7-bis(Dimethylamino)phenazathionium chloride, Basic Blue 9, Tetramethylthionine chloride

  • CAS Number 122965-43-9

  • Empirical Formula (Hill Notation) C16H18ClN3S · xH2O

  • Molecular Weight 319.85 (anhydrous basis)

  •  Beilstein Registry Number 3599847

  •  Colour Index Number 52015

  •  EC Number 200-515-2

  •  MDL number MFCD00150006

  •  PubChem Substance ID 24897317

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Properties

Related Categories Cell Biology, Certified Stains & Dyes, Core Bioreagents, Hematology and Histology, Life Science Reagents for DNA/RNA Electrophoresis,
grade   certified by the Biological Stain Commission
InChI Key   WQVSELLRAGBDLX-UHFFFAOYSA-M
form   powder
composition   Dye content, ≥82%
solubility   H2O: 4mg/4ml
Gene Information   human ... CYP1A2(1544), GSR(2936)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

25, 100 g in glass bottle

Suitability

Certified for use in Mallory′s phloxine-methylene blue stain; Levine & Black stain for bacteria in milk; Wright stain for blood smears.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
SP5740000

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the solubility of Methylene blue, Product M9140?
The product is soluble in water at 50 mg/mL, EGME at 60 mg/mL and ethanol at 70 mg/mL. EGME is an abbreviation for ethylene glycol monomethyl ether, also known as 2-methoxyethanol or methyl cellosolve.
How can Methylene blue, Product M9140, be used?
Probably its most important application is as an in vitro diagnostic bacteriology, cytology, hematology, and histology stain. Methylene Blue serves as a precursor to the Methylene Azures and as a component of the various Ramanowsky-type blood stains (Wright, Leishman, Giemsa and others). While pure Methylene Blue is not a good nuclear stain, it readily undergoes oxidative demethylation to form the various Methylene Azures, which are good nuclear stains.
How is dye content determined for Methylene blue, Product M9140?
Dye content is determined spectrophotometrically by noting the dye's absorbance peak, which is typically near 665 nm, or by calculating the ratio of the absorbances at 650 nm over 680 nm. (The dye exhibits smaller peaks at approximately 292 and 246 nm).
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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