EMAIL THIS PAGE TO A FRIEND
M9140 Sigma-Aldrich

Methylene blue

certified by the Biological Stain Commission

Synonym: 3,7-bis(Dimethylamino)phenazathionium chloride, Basic Blue 9, Tetramethylthionine chloride

  • CAS Number 122965-43-9

  • Empirical Formula (Hill Notation) C16H18ClN3S · xH2O

  • Molecular Weight 319.85 (anhydrous basis)

  •  Colour Index Number 52015

  •  Beilstein Registry Number 3599847

  •  EC Number 200-515-2

  •  MDL number MFCD00150006

  •  PubChem Substance ID 24897317

Purchase

Properties

Related Categories Cell Biology, Certified Stains & Dyes, Core Bioreagents, Hematology and Histology, Life Science Reagents for DNA/RNA Electrophoresis,
grade   certified by the Biological Stain Commission
InChI Key   WQVSELLRAGBDLX-UHFFFAOYSA-M
form   powder
composition   Dye content, ≥82%
solubility   H2O: 4mg/4ml
Gene Information   human ... CYP1A2(1544), GSR(2936)

Description

Packaging

25, 100 g in glass bottle

Suitability

Certified for use in Mallory′s phloxine-methylene blue stain; Levine & Black stain for bacteria in milk; Wright stain for blood smears.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
SP5740000

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the solubility of Methylene blue, Product M9140?
The product is soluble in water at 50 mg/mL, EGME at 60 mg/mL and ethanol at 70 mg/mL. EGME is an abbreviation for ethylene glycol monomethyl ether, also known as 2-methoxyethanol or methyl cellosolve.
How canMethylene blue, Product M9140, be used?
Probably its most important application is as an in vitro diagnostic bacteriology, cytology, hematology, and histology stain. Methylene Blue serves as a precursor to the Methylene Azures and as a component of the various Ramanowsky-type blood stains (Wright, Leishman, Giemsa and others). While pure Methylene Blue is not a good nuclear stain, it readily undergoes oxidative demethylation to form the various Methylene Azures, which are good nuclear stains.
How is dye content determined for Methylene blue, Product M9140?
Dye content is determined spectrophotometrically by noting the dye's absorbance peak, which is typically near 665 nm, or by calculating the ratio of the absorbances at 650 nm over 680 nm. (The dye exhibits smaller peaks at approximately 292 and 246 nm).
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions

LC-MS Grade Solvents and Reagents

Wired Weeks Savings
Protocols & Articles
Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?