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N5514 Sigma-Aldrich

Nitro Blue Tetrazolium

tablet

Synonym: Nitrotetrazolium Blue Chloride

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Properties

Related Categories Alkaline Phosphatase Substrates for Western Blotting, Alphabetical Listing, Biochemicals and Reagents, Colorimetric, Core Bioreagents,
Quality Level   200
form   tablet
application(s)   hematology: suitable
  histology: suitable
pH   4.5-5.5
solubility   soluble, clear, yellow
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   2-8°C
SMILES string   [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
InChI   1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key   FSVCQIDHPKZJSO-UHFFFAOYSA-L

Description

General description

Nitro Blue Tetrazolium is a tetrazole compound with two tetrazole rings.

Application

Nitro Blue Tetrazolium has been used:
• to measure superoxide radicals
• in lactate dehydrogenase quantification assay
• for blot development in western blotting analysis
• in NADPH-d histochemistry
• as a reagent to analyse the properties of murine intestinal mucins by electrophoresis and histology
• in immunohistochemistry

Packaging

10, 25 tablets in glass bottle

Quantity

Contains 10 mg substrate per tablet.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Peer-Reviewed Papers
15

References

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