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N6639 Sigma-Aldrich

Nitrotetrazolium Blue chloride

powder, electrophoresis grade

Synonym: 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

  • CAS Number 298-83-9

  • Linear Formula C40H30N10O6 · 2Cl

  • Molecular Weight 817.64

  •  Beilstein/REAXYS Number 4115923

  •  EC Number 206-067-4

  •  MDL number MFCD00012159

  •  PubChem Substance ID 24897786

  •  NACRES NA.52

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Properties

Related Categories Cell Biology, Colorimetric, Hematology and Histology, Molecular Biology, N,
Quality Level   100
grade   electrophoresis grade
assay   >90%
form   powder
solubility   H2O: 10 mg/mL (A stock solution at 10mg/ml in water is stable for 1-2 weeks in the dark at 0-4°C.)
  2-methoxyethanol: 20 mg/mL
  ethanol: 5 mg/mL
storage temp.   2-8°C
SMILES string   [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
InChI   1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key   FSVCQIDHPKZJSO-UHFFFAOYSA-L

Description

General description

Nitrotetrazolium Blue chloride (NBT) is a dye that is used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).

Application

Nitrotetrazolium Blue chloride has been used:
• to determine the superoxide anion in skeletal muscle cells
• as a component in Tris-HCl buffered saline for nicotinamide adenine dinucleotide phosphate (NADPH)-diaphorase histochemistry of brain sections
• as a chromogenic substrate to study the histochemical detection of hydrogen peroxide (H2O2) and superoxide (O2- ) anion in Brassica juncea seedlings
• as a component in Triton X-100 solution to perform staining of coronal brain sections

Packaging

1 g in glass bottle

50, 250 mg in glass insert

Biochem/physiol Actions

Nitrotetrazolium Blue chloride (NBT) has also been used as a colorimetric indicator of bacterial infection in blood samples. NBT has also been used as a redox indicator for other enzymatic reactions. NBT produces a dark blue insoluble formazan compound while reacting with O2.

Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Danger
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
XF8045000
Protocols & Articles

Articles

Intro to Detection Methods Used in Molecular Biology

Different detection methods are used to visualize the nucleic acids and specific proteins transferred to membrane support. Southern and northern blotting involves the use of radioactive probes and th...
Keywords: Catalytic combustion detector, Detection methods, Northern blot, Nucleic acid hybridization, Purification, Size-exclusion chromatography, Western blot

Protocols

Nitrotetrazolium blue chloride for molecular biology protocol

CAS RN 298-83-9 EC 206-067-4 Synonyms: p-Nitro-Blue tetrazolium chloride, p-nitrotetrazolium blue, NBT, Nitro BT
Keywords: Extinction coefficient, Indicators, Melting, Western blot

Peer-Reviewed Papers
15

References

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I9516 2-Propanol, BioReagent, for molecular biology, ≥99.5%

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