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N6876 Sigma-Aldrich

Nitrotetrazolium Blue chloride

≥90.0% (HPLC)

Synonym: 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT

  • CAS Number 298-83-9

  • Linear Formula C40H30N10O6 · 2Cl

  • Molecular Weight 817.64

  •  Beilstein/REAXYS Number 4115923

  •  MDL number MFCD00012159

  •  PubChem Substance ID 24897795

  •  NACRES NA.47

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Properties

Related Categories Alkaline Phosphatase Substrates for Western Blotting, Alphabetical Listing, Biochemicals and Reagents, Cell Biology, Colorimetric,
Quality Level   200
assay   ≥90.0% (HPLC)
form   powder
application(s)   hematology: suitable
  histology: suitable
impurities   ≤10% solvent
solubility   H2O: 10 mg/mL
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   2-8°C
SMILES string   [Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccccc4)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccccc8
InChI   1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key   FSVCQIDHPKZJSO-UHFFFAOYSA-L

Description

Application

• Nitrotetrazolium Blue chloride has been used to detect the generation of oxygen metabolites in cells.
• It has been used as a substrate for western blot detection.
• It has been used for the staining of histological tissue sections.

Packaging

1, 5 g in glass bottle

10×10, 50, 100, 250, 500 mg in glass insert

Biochem/physiol Actions

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridized to its target.

Substrates

Substrate for dehydrogenases and other oxidases.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Target organs 
Eyes
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

Protocols

Nitrotetrazolium blue chloride for molecular biology protocol

CAS RN 298-83-9 EC 206-067-4 Synonyms: p-Nitro-Blue tetrazolium chloride, p-nitrotetrazolium blue, NBT, Nitro BT
Keywords: Extinction coefficient, Indicators, Melting, Western blot

Peer-Reviewed Papers
15

References

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