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P6585 Sigma-Aldrich

TWEEN® 20

Low-peroxide; Low-carbonyls

Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Culture, Core Bioreagents, Detergents,
description   non-ionic
form   viscous liquid
mol wt   ~1228
contains   BHT as antioxidant
impurities   ≤0.5 μmol/g Peroxides
  ≤1.0 μmol/g Carbonyls
  ≤3.0% water
refractive index   n20/D 1.468 (lit.)
CMC   0.06 mM (20-25°C)
transition temp   cloud point 76 °C
density   1.095 g/mL at 25 °C (lit.)
  1.1 g/cm3 at 25 °C (lit.)
HLB   16.7
storage temp.   2-8°C

Description

General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

Tween 20 has been used along with PBS to wash plates in ELISA technique.

Non-ionic detergent

Packaging

10, 100 mL in glass bottle

Preparation Note

Purified to remove peroxides and aldehydes

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TR7400000
Flash Point(F) 
>230 °F
Flash Point(C) 
>110 °C

Documents

Certificate of Analysis

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Certificate of Origin

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Single- & multi-channel pipettes from BRAND

Setting the Standard for Solid Formulation
Protocols & Articles
Peer-Reviewed Papers
15

References

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