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P7170 Sigma-Aldrich

Ponceau S solution

BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid

  • CAS Number 6226-79-5

  • Empirical Formula (Hill Notation) C22H12N4Na4O13S4

  • Molecular Weight 760.57

  •  MDL number MFCD00003892

  •  PubChem Substance ID 24898827

Purchase

Properties

Related Categories Buffers, Reagents, & Stains, Cell Biology, Core Bioreagents, General Reagents, Hematology and Histology,
Quality Level   200
product line   BioReagent
form   liquid
concentration   0.1 % (w/v) in 5% acetic acid
application(s)   hematology: suitable
  histology: suitable
density   1.01 g/cm3 at 20 °C
suitability   suitable for electrophoresis
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   [Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O
InChI   1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;
InChI key   KQHKSGRIBYJYFX-WNDMICSESA-J

Description

General description

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Application

Ponceau S solution has been used for membrane staining (Western blotting).

Packaging

1 L in poly bottle

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

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Introduction to Western Blotting

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Peer-Reviewed Papers
15

References

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