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P8341 Sigma-Aldrich

TWEEN® 20

Low-peroxide; Low-carbonyls

Synonym: Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate

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Properties

Related Categories Antigen-Vaccine Preparation, Biochemicals and Reagents, Cell Biology, Cell Culture, Cell Lysis Reagents,
description   non-ionic
form   viscous liquid
mol wt   ~1228
impurities   ≤0.5 μmol/g Peroxides
  ≤1.0 μmol/g Carbonyls
  ≤3.0% water
refractive index   n20/D 1.468 (lit.)
CMC   0.06 mM (20-25°C)
transition temp   cloud point 76 °C
density   1.095 g/mL at 25 °C (lit.)
  1.1 g/cm3 at 25 °C (lit.)
HLB   16.7
storage temp.   −20°C

Description

General description

Tween®20, is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 is widely used in several biochemical applications. It has been used:
• As an emulsifying agent for the preparation of stable oil-in-water emulsions
• In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
• As a blocking agent for membrane based immunoassays at a typical concentration of 0.05%
• For lysing mammalian cells at a concentration of 0.005 to 0.5%

Non-ionic detergent

Packaging

10, 5×10 mL in glass bottle

Other Notes

Preservative Free

Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TR7400000
Flash Point(F) 
>230 °F
Flash Point(C) 
>110 °C

Documents

Certificate of Analysis

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Certificate of Origin

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Single- & multi-channel pipettes from BRAND

Setting the Standard for Solid Formulation
Protocols & Articles
Peer-Reviewed Papers
15

References

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