PHR1002 Supelco


Pharmaceutical Secondary Standard; Certified Reference Material

Synonym: D-(−)-Fructose, D-Levulose, Fruit sugar

  • CAS Number 57-48-7

  • Empirical Formula (Hill Notation) C6H12O6

  • Molecular Weight 180.16

  •  Beilstein/REAXYS Number 1239004

  •  EC Number 200-333-3

  •  MDL number MFCD00148910

  •  PubChem Substance ID 329823033

  •  NACRES NA.24



Related Categories Analytical Standards, Analytical/Chromatography, Carbohydrate Standards, E-J, Food & Beverage Standards,
Quality Level   300
grade   certified reference material
  pharmaceutical secondary standard
form   neat
analyte chemical class(es)   oligosaccharides
application(s)   HPLC: suitable
  gas chromatography (GC): suitable
mp   119-122 °C (dec.) (lit.)
Featured Industry   Cleaning Products
Food and Beverages
Personal Care
Pharmaceutical (small molecule)
format   neat
pharmacopeia traceability   traceable to PhEur F0550000
  traceable to USP 1286504
storage temp.   room temp
SMILES string   OC[C@@H](O)[C@@H](O)[C@H](O)C(=O)CO
InChI   1S/C6H12O6/c7-1-3(9)5(11)6(12)4(10)2-8/h3,5-9,11-12H,1-2H2/t3-,5-,6-/m1/s1


General description

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.


Fructose may be used as a pharmaceutical reference standard for the determination of the analyte in pharmaceutical formulations by spectrophotometry.

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.

Biochem/physiol Actions

A monosaccharide in sweet fruits and honey that is soluble in water, alcohol, or ether. It is used as a preservative and an intravenous infusion in parenteral feeding.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP (PhEur) and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.


To see an example of a Certificate of Analysis for this material enter LRAC0254 in the slot below. This is an example certificate only and may not be the lot that you receive.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles


Determination of Additives in Beverages Using Ascentis Express Columns

Beverages, such as sodas and energy drinks, can contain a number of polar ingredients, which are easily soluble in the water matrix of the drinks. These ingredients include sweeteners (sugars and sug...
Olga Shimelis, Hugh Cramer, and Michael D. Buchanan
US Reporter Volume 30.3
Keywords: Chromatography, High performance liquid chromatography, Mass spectrometry, Sample preparations, Separation, Vitamins

HPLC Analysis of Sugars and Sugar Enantiomers on Astec® CYCLOBOND® I 2000

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Sugars and Sugar Enantiomers on Astec® CYCLOBOND® I 2000
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Sugars on SUPELCOSIL™ LC-NH2
Keywords: Chromatography, High performance liquid chromatography

Peer-Reviewed Papers


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