PHR1020 Supelco


Pharmaceutical Secondary Standard; Certified Reference Material

Synonym: 1,2,3-Propanetriol, Glycerin, Glycerol

  • CAS Number 56-81-5

  • Linear Formula HOCH2CH(OH)CH2OH

  • Molecular Weight 92.09

  •  Beilstein/REAXYS Number 635685

  •  EC Number 200-289-5

  •  MDL number MFCD00004722

  •  PubChem Substance ID 329823051

  •  NACRES NA.24



Related Categories Alcohol, All Alphabetically Sorted, Analytical Standards, Analytical/Chromatography, Chinese Food Contact Materials Regulation GB 9685 2016,
Quality Level   300
grade   certified reference material
  pharmaceutical secondary standard
vapor density   3.1 (vs air)
vapor pressure   <1 mmHg ( 20 °C)
form   neat
autoignition temp.   698 °F
application(s)   HPLC: suitable
  gas chromatography (GC): suitable
refractive index   n20/D 1.474 (lit.)
bp   182 °C/20 mmHg (lit.)
mp   20 °C (lit.)
density   1.25 g/mL (lit.)
Featured Industry   Cleaning Products
Flavors and Fragrances
Food and Beverages
Personal Care
Pharmaceutical (small molecule)
format   neat
pharmacopeia traceability   traceable to PhEur G0400000
  traceable to USP 1295607
SMILES string   OCC(O)CO
InChI   1S/C3H8O3/c4-1-3(6)2-5/h3-6H,1-2H2


General description

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.


Glycerin may be used as a pharmaceutical reference standard for the determination of the analyte in pharmaceutical formulations and cosmetics by chromatography techniques.

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.

Glycerol is used both in sample preparation and gel formation for polyacrylamide gel electrophoresis. Glycerol (5-10%) increases the density of a sample so that the sample will layer at the bottom of a gel’s sample well. Glycerol is also used to aid in casting gradient gels and as a protein stabilizer and storage buffer component.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP (PhEur) and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.


To see an example of a Certificate of Analysis for this material enter LRAB7812 in the slot below. This is an example certificate only and may not be the lot that you receive.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
390.2 °F - Pensky-Martens closed cup
Flash Point(C) 
199 °C - Pensky-Martens closed cup
Protocols & Articles


ASTM D6584: GC Analysis of Glycerin Impurity in B100 Biodiesel on MET-Biodiesel

From our library of Articles, Sigma-Aldrich presents ASTM D6584: GC Analysis of Glycerin Impurity in B100 Biodiesel on MET-Biodiesel
Keywords: Chromatography, Flame ionization detector, Gas chromatography, Purification

GC Analysis of Glycols on Nukol™

From our library of Articles, Sigma-Aldrich presents GC Analysis of Glycols on Nukol™
Keywords: Chromatography, Flame ionization detector, Gas chromatography, Purification

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Flavor and Fragrance Analysis

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