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Cholecalciferol (Vitamin D3)

Pharmaceutical Secondary Standard; Certified Reference Material

Synonym: (+)-Vitamin D3, 7-Dehydrocholesterol activated, Activated 7-dehydrocholesterol, Calciol, Cholecalciferol

  • CAS Number 67-97-0

  • Empirical Formula (Hill Notation) C27H44O

  • Molecular Weight 384.64

  •  Beilstein/REAXYS Number 2339331

  •  EC Number 200-673-2

  •  MDL number MFCD00078131

  •  PubChem Substance ID 329823242

  •  NACRES NA.24

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Properties

Related Categories Analytical Standards, Analytical/Chromatography, C-D, Food and Cosmetic Component Standards, Pharmaceutical Secondary Standards,
Quality Level   300
grade   certified reference material
  pharmaceutical secondary standard
form   neat
application(s)   HPLC: suitable
  gas chromatography (GC): suitable
mp   83-86 °C (lit.)
Featured Industry   Cleaning Products
Cosmetics
Food and Beverages
Personal Care
Pharmaceutical (small molecule)
format   neat
pharmacopeia traceability   traceable to BP 787
  traceable to PhEur C2100000
  traceable to USP 1131009
SMILES string   CC(C)CCC[C@@H](C)[C@@]1([H])CC[C@@]([C@]1(C)CCC/2)([H])C2=C\C=C(C[C@@H](O)CC3)/C3=C
InChI   1S/C27H44O/c1-19(2)8-6-9-21(4)25-15-16-26-22(10-7-17-27(25,26)5)12-13-23-18-24(28)14-11-20(23)3/h12-13,19,21,24-26,28H,3,6-11,14-18H2,1-2,4-5H3/b22-12+,23-13-/t21-,24+,25-,26+,27-/m1/s1
InChI key   QYSXJUFSXHHAJI-YRZJJWOYSA-N
Gene Information   human ... VDR(7421)

Description

General description

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.
Cholecalciferol (vitamin D3) is a fat-soluble vitamin that manifests skeletal and extra-skeletal effects. The skeletal effects augment the bone health whereas the extra-skeletal effects concern the immune system, the cognition, the cardiovascular system, etc.

Application

Cholecalciferol (Vitamin D3) may be used as a pharmaceutical reference standard for the determination of the analyte in food, feeds, serum samples and pharmaceutical formulations by various chromatography techniques.

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.

Biochem/physiol Actions

Vitamin D acts through a receptor that is a member of the ligand-dependent transcription factor superfamily. Modulates the proliferation and differentiation of both normal and cancer cells. Has antiproliferative and antimetastatic effects on breast, colon, and prostate cancer cells. Activated vitamin D receptors in intestine and bone maintain calcium absorbance and homeostasis.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP (PhEur) and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

Footnote

To see an example of a Certificate of Analysis for this material enter LRAC0270 in the slot below. This is an example certificate only and may not be the lot that you receive.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 2811 6.1 / PGII
WGK Germany 
WGK 2
RTECS 
VS2900000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D in Infant formula on Titan™ C18

From our library of Articles, Sigma-Aldrich presents AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D in Infant formula on Titan™ C18
Keywords: High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Saponifications, Vitamins

AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D in Milk on Titan™ C18

From our library of Articles, Sigma-Aldrich presents AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D in Milk on Titan™ C18
Keywords: Chromatography, Mass spectrometry, Vitamins

AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D2 and Vitamin D3 on Titan™ C18

From our library of Articles, Sigma-Aldrich presents AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D2 and Vitamin D3 on Titan™ C18
Keywords: Mass spectrometry, Separation, Vitamins

HPLC Analysis Report of Vitamin D2 and D3 on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis Report of Vitamin D2 and D3 on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

HPLC Analysis of Bufalin Toxin on Discovery® BIO Wide Pore C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Bufalin Toxin on Discovery® BIO Wide Pore C18
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si, 15 cm Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si, 15 cm Column
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fat Soluble Vitamins by Normal Phase Chromatography on Ascentis® Si
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Acetonitrile Mobile Phase

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Acetonitrile Mobile Phase
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Methanol Mobile Phase

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Methanol Mobile Phase
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Fat Soluble Vitamins on Ascentis® RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fat Soluble Vitamins on Ascentis® RP-Amide
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Fat-Soluble Vitamins on Ascentis® Express C18, Methanol Mobile Phase

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fat-Soluble Vitamins on Ascentis® Express C18, Methanol Mobile Phase
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Vitamins, Fat Soluble (D2 and D3), on Discovery® HS C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamins, Fat Soluble (D2 and D3), on Discovery® HS C18
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Vitamins, Fat Soluble, on Ascentis® Si

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamins, Fat Soluble, on Ascentis® Si
Keywords: Chromatography, High performance liquid chromatography, Vitamins

LC/MS Analysis of Vitamin D2, D3, Gamma- and Alpha-Tocopherol on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Vitamin D2, D3, Gamma- and Alpha-Tocopherol on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Mass spectrometry, Vitamins

Peer-Reviewed Papers
15

References

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