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Ergocalciferol (Vitamin D2)

Pharmaceutical Secondary Standard; Certified Reference Material

Synonym: Calciferol, Ercalciol, Ergocalciferol, Ergosterol irradiated, Irradiated ergosterol, Vitamin D2

  • CAS Number 50-14-6

  • Empirical Formula (Hill Notation) C28H44O

  • Molecular Weight 396.65

  •  Beilstein/REAXYS Number 1916682

  •  EC Number 200-014-9

  •  MDL number MFCD00166988

  •  PubChem Substance ID 329823243

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Properties

Related Categories Analytical Standards, Analytical/Chromatography, E-J, Food and Cosmetic Component Standards, Pharmaceutical Secondary Standards,
Quality Level   300
grade   certified reference material
  pharmaceutical secondary standard
form   neat
application(s)   HPLC: suitable
  gas chromatography (GC): suitable
mp   114-118 °C (lit.)
Featured Industry   Cosmetics
Pharmaceutical (small molecule)
format   neat
pharmacopeia traceability   traceable to BP 788
  traceable to PhEur E0900000
  traceable to USP 1239005
storage temp.   no temp limit
SMILES string   CC(C)[C@@H](C)/C=C/[C@@H](C)[C@@]1([H])CC[C@@]([C@]1(C)CCC/2)([H])C2=C\C=C(C[C@@H](O)CC3)/C3=C
InChI   1S/C28H44O/c1-19(2)20(3)9-10-22(5)26-15-16-27-23(8-7-17-28(26,27)6)12-13-24-18-25(29)14-11-21(24)4/h9-10,12-13,19-20,22,25-27,29H,4,7-8,11,14-18H2,1-3,5-6H3/b10-9+,23-12+,24-13-/t20-,22+,25-,26+,27-,28+/m0/s1
InChI key   MECHNRXZTMCUDQ-RKHKHRCZSA-N
Gene Information   human ... VDR(7421)

Description

General description

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

Application

Ergocalciferol (Vitamin D2) may be used as a pharmaceutical reference standard for the determination of the analyte in serum samples, cosmetics and pharmaceutical formulations by various chromatography techniques.

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP (PhEur) and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

Footnote

To see an example of a Certificate of Analysis for this material enter LRAB7854 in the slot below. This is an example certificate only and may not be the lot that you receive.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 2811 6.1 / PGII
WGK Germany 
WGK 3
RTECS 
KE1050000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D2 and Vitamin D3 on Titan™ C18

From our library of Articles, Sigma-Aldrich presents AOAC Method 2011.11: UHPLC/MS/MS Analysis of Vitamin D2 and Vitamin D3 on Titan™ C18
Keywords: Mass spectrometry, Separation, Vitamins

HPLC Analysis Report of Vitamin D2 and D3 on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis Report of Vitamin D2 and D3 on Ascentis® Express C18
Keywords: Chromatography, Clinical, High performance liquid chromatography, Vitamins

HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Acetonitrile Mobile Phase

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Acetonitrile Mobile Phase
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Methanol Mobile Phase

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fat Soluble Vitamins on Ascentis® Express RP-Amide, Methanol Mobile Phase
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Fat-Soluble Vitamins on Ascentis® Express C18, Methanol Mobile Phase

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fat-Soluble Vitamins on Ascentis® Express C18, Methanol Mobile Phase
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Vitamins, Fat Soluble (D2 and D3), on Discovery® HS C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Vitamins, Fat Soluble (D2 and D3), on Discovery® HS C18
Keywords: Chromatography, High performance liquid chromatography, Vitamins

Peer-Reviewed Papers
15

References

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