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PHR1353 Supelco

Estradiol

Pharmaceutical Secondary Standard; Certified Reference Material

Synonym: β-Estradiol, 1,3,5-Estratriene-3,17β-diol, 17β-Estradiol, 3,17β-Dihydroxy-1,3,5(10)-estratriene, Dihydrofolliculin

  • CAS Number 50-28-2

  • Empirical Formula (Hill Notation) C18H24O2

  • Molecular Weight 272.38

  •  Beilstein/REAXYS Number 1914275

  •  EC Number 200-023-8

  •  MDL number MFCD00003693

  •  PubChem Substance ID 329823337

  •  NACRES NA.24

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Properties

Related Categories Analytical Standards, Analytical/Chromatography, E-J, Pharmaceutical Secondary Standards, Pharmacopeia & Metrological Institutes Standards More...
Quality Level   300
grade   certified reference material
  pharmaceutical secondary standard
form   neat
application(s)   HPLC: suitable
  gas chromatography (GC): suitable
mp   176-180 °C (lit.)
Featured Industry   Pharmaceutical (small molecule)
format   neat
pharmacopeia traceability   traceable to BP 729
  traceable to PhEur E1620000
  traceable to USP 1250008
SMILES string   O[C@H]1CC[C@@]2([H])[C@]3([H])CCC4=CC(O)=CC=C4[C@@]3([H])CC[C@@]21C
InChI   1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17+,18+/m1/s1
InChI key   VOXZDWNPVJITMN-ZBRFXRBCSA-N
Gene Information   human ... ESR1(2099)

Description

General description

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.
Estradiol is a steroid hormone present naturally in the human body. The availability of this hormone is quintessential in older women as it is a determinant of menopausal syndromes. It is utilized for the prevention of cardiovascular diseases.

Application

Estradiol may be used as a pharmaceutical reference standard for the determination of the analyte in pharmaceutical formulations by various chromatography techniques.

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.

Biochem/physiol Actions

The major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female phenotype in embryogenesis and during puberty by regulating gene transcription and, thus, protein synthesis. It also induces the production of gonadotropins which, in turn, induce ovulation. Exposure to estradiol increases breast cancer incidence and proliferation.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP (PhEur) and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

Footnote

To see an example of a Certificate of Analysis for this material enter LRAC3704 in the slot below. This is an example certificate only and may not be the lot that you receive.

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

HPLC Analysis of Estrogens on Ascentis® Express Biphenyl

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Estrogens on Ascentis® Express Biphenyl
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Steroids on Ascentis® C18 (Mobile phase: 55:45)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Steroids on Ascentis® C18 (Mobile phase: 55:45)
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Analysis of Steroids on Ascentis® Express Biphenyl

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Steroids on Ascentis® Express Biphenyl
Keywords: Chromatography, High performance liquid chromatography

HPLC Analysis of Steroids on Ascentis® RP-Amide (Mobile phase: 55:45)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Steroids on Ascentis® RP-Amide (Mobile phase: 55:45)
Keywords: Chromatography, Clinical, High performance liquid chromatography

Hyper-Fast Separations on Ascentis® Express 2.7 μm at Half the Pressures of sub-2 μm Columns

From our library of Articles, Sigma-Aldrich presents Hyper-Fast Separations on Ascentis® Express 2.7 μm at Half the Pressures of sub-2 μm Columns
Keywords: Chromatography, High performance liquid chromatography, Pharmaceutical

UHPLC Analysis of Conjugated Estrogens on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Conjugated Estrogens on Ascentis® Express C18
Keywords: Chromatography, Clinical, Solvents

UHPLC Analysis of Conjugated Estrogens on Titan™ C18

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Conjugated Estrogens on Titan™ C18
Keywords: Chromatography, Clinical, Solvents

UHPLC/MS Analysis of Estradiols using Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents UHPLC/MS Analysis of Estradiols using Ascentis® Express C18
Keywords: Mass spectrometry

Peer-Reviewed Papers
15

References

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