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HEPES

Pharmaceutical Secondary Standard; Certified Reference Material

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, HEPES, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein/REAXYS Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 329822594

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Properties

Related Categories A-H, Additional Standards, Analytical Standards, Analytical/Chromatography, Chromatography,
grade   certified reference material
  pharmaceutical secondary standard
InChI Key   JKMHFZQWWAIEOD-UHFFFAOYSA-N
pH   5.0-6.5 (25 °C, 238 g/L)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
Featured Industry   Pharmaceutical (small molecule)
format   neat

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

Application

HEPES has been used as a component of homogenization buffer to homogenize adipose tissue for liquid chromatography/mass spectrometry. It has also been used in HN buffer for dissolving viral stock titer after dot blot hybridization.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

Footnote

To see an example of a Certificate of Analysis for this material enter LRAA2736 in the slot below. This is an example certificate only and may not be the lot that you receive.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TL6809000

Documents

Certificate of Analysis

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Protocols & Articles
Peer-Reviewed Papers
15

References

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