PHR1533 Supelco


Pharmaceutical Secondary Standard; Certified Reference Material

Synonym: 3β-Hydroxy-5-cholestene, 5-Cholesten-3β-ol, Cholesterol

  • CAS Number 57-88-5

  • Empirical Formula (Hill Notation) C27H46O

  • Molecular Weight 386.65

  •  Beilstein/REAXYS Number 1915888

  •  EC Number 200-353-2

  •  MDL number MFCD00003646

  •  PubChem Substance ID 329823445

  •  NACRES NA.24



Related Categories Analytical Standards, Analytical/Chromatography, C-D, Pharmaceutical Secondary Standards, Pharmacopeia & Metrological Institutes Standards More...
Quality Level   300
grade   certified reference material
  pharmaceutical secondary standard
form   powder
packaging   pkg of 500 mg
application(s)   HPLC: suitable
  gas chromatography (GC): suitable
bp   360 °C (lit.)
mp   147-149 °C (lit.)
solubility   water: soluble 0.00003 g/L at 20 °C
density   1.067 g/mL at 25 °C (lit.)
Featured Industry   Pharmaceutical (small molecule)
format   neat
pharmacopeia traceability   traceable to PhEur C2155000
  traceable to USP 1131960
SMILES string   CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C
InChI   1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1


General description

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards. Cholesterol is a waxy material that belongs to the class of sterol-lipids found naturally in foods from animal sources, such as egg yolks, meat, and cheese. It serves as a precursor for the biosynthesis of steroid hormones, bile acid and vitamin D.


Cholesterol may be used as a pharmaceutical reference standard for the determination of the analyte in pharmaceutical formulations and food products by gas chromatography, or by high performance liquid chromatography.

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.

Biochem/physiol Actions

Major component of all biological membranes; ~25% of total brain lipid is cholesterol.

Analysis Note

These secondary standards offer multi-traceability to the USP, EP and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.


To see an example of a Certificate of Analysis for this material enter LRAC0249 in the slot below. This is an example certificate only and may not be the lot that you receive.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


GC Analysis of Sterols (Silylated Derivatives) in Olive Oil (Free Sterol Fraction) on SLB®-5ms after SPE using Discovery DSC-Si

From our library of Articles, Sigma-Aldrich presents GC Analysis of Sterols (Silylated Derivatives) in Olive Oil (Free Sterol Fraction) on SLB®-5ms after SPE using Discovery DSC-Si
Keywords: Agriculture, Calorimetry, Chromatography, Fractionation, Gas chromatography, Gas chromatography mass spectrometry, Mass selective detector, Mass spectrometry, Purification, Sample preparations, Saponifications, Silylations, Solid phase extractions

Peer-Reviewed Papers


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