PHR1614 Supelco


Pharmaceutical Secondary Standard; Certified Reference Material

Synonym: 1-Ethyl-6-fluoro-1,4-dihydro-4-oxo-7-(1-piperazinyl)-3-quinolinecarboxylic acid, 1-Ethyl-6-fluoro-1,4-dihydro-4-oxo-7-piperazino-3-quinolinecarboxylic acid, Norfloxacin



Related Categories Additional Standards, Analytical Standards, Analytical/Chromatography, Chromatography, I-Z,
grade   certified reference material
  pharmaceutical secondary standard
packaging   pkg of 1 g
Featured Industry   Pharmaceutical (small molecule)
format   neat
pharmacopeia traceability   traceable to BP 670
  traceable to PhEur N1230000
  traceable to USP 1471506


General description

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.
Norfloxacin is a synthetic broad-spectrum antibacterial drug that is effective against various gram positive and gram negative bacteria. Its major application is in the treatment of urinary tract infections.


Norfloxacin may be used as a pharmaceutical reference standard for the determination of the analyte in pharmaceutical formulations and serum samples by high performance liquid chromatography (HPLC) and spectrophotometric methods.

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.

Biochem/physiol Actions

Norfloxacin blocks DNA replication by interfering with an ATP-induced structural transition of DNA complexed with DNA gyrase (topoisomerase).
Mode of action: inhibits bacterial DNA replication
Antimicrobial spectrum: Gram-negative bacteria; less effective against Gram-positive bacteria

Analysis Note

These secondary standards offer multi-traceability to the USP, EP and BP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.


To see an example of a Certificate of Analysis for this material enter LRAC2802 in the Documents slot below. This is an example certificate only and may not be the lot that you receive.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

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Protocols & Articles


HPLC Analysis of Antibacterials/Antifungals on Ascentis® RP-Amide, Phosphate Mobile Phase

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Antibacterials/Antifungals on Ascentis® RP-Amide, Phosphate Mobile Phase
Keywords: Antibiotics, Antifungals, Chromatography, High performance liquid chromatography, Pharmaceutical

HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis® Phenyl

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis® Phenyl
Keywords: Antibiotics, Chromatography, High performance liquid chromatography, Mass spectrometry, Pharmaceutical

Peer-Reviewed Papers


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